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PriSM is a set of algorithms designed specifically to create degenerate primers for the amplification and sequencing of short viral genomes while maintaining sample population diversity.

To download PriSM click here.

Yu Q, Ryan EM, Allen TM, Birren BW, Henn MR, Lennon NJ (2011) PriSM: a primer selection and matching tool for amplification and sequencing of viral genomes. Bioinformatics 27(2): 266-267.

Support for this tool was provided by the National Institute of Allergy and Infectious Diseases through the Microbial Sequencing Center and Genome Sequencing Center for Infectious Diseases, by grants made by the Bill & Melinda Gates Foundation (awarded to the Ragon Institute), the Gordon & Betty Moore Foundation, and through internal support at the Broad Institute.