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BroadE: CRISPR in a nutshell

This fall 2017 BroadE workshop covered:

  1. Introduction to CRISPR-Cas systems and basic genome editing methodologies (focusing on Cas9), including guide design, in vitro and in vivo delivery, and assaying results
  2. Guide to CRISPR-Cas9-based screening applications (both loss- and gain-of-function)
  3. Overview of frontiers in genome editing, including therapeutic delivery modes, approaches to enhancing specificity, and novel RNA-guided RNA-targeting enzymes