MitoCarta2.0 is an inventory of 1158 human and mouse genes encoding proteins with strong support of mitochondrial localization. To generate this inventory, we performed mass spectrometry of mitochondria isolated from fourteen tissues, assessed protein localization through large-scale GFP tagging/microscopy, and integrated these results with six other genome-scale datasets of mitochondrial localization, using a Bayesian approach. MitoCarta2.0, released 2015, is an update from the MitoCarta1.0 inventory published in Cell, 2008.
Citation:
- Calvo, S.E., Klauser, C.R., Mootha, V.K. MitoCarta2.0: an updated inventory of mammalian mitochondrial proteins (2015). Nucleic Acids Research [doi: 10.1093/nar/gkv1003]
- Pagliarini, D.J., Calvo, S.E., Chang, B., Sheth, S.A., Vafai, S.B., Ong, S.E., Walford, G.A., Sugiana, C., Boneh, A., Chen, W.K., et al. (2008). A mitochondrial protein compendium elucidates complex I disease biology. Cell 134, 112-123. [Pubmed: 18614015]
New! For location of mitochondrial targeting signals, see Mito_Ntermini.xlsx -- created via N-terminal proteomics from mouse liver mitochondria [Pubmed: 28122942].
View MitoCarta2.0 datasets
| Human MitoCarta2.0 | 1158 MitoCarta genes: evidence of mitochondrial protein localization and protein distribution across 14 tissues | |
| Mouse MitoCarta2.0 | 1158 MitoCarta genes: evidence of mitochondrial protein localization and protein distribution across 14 tissues | |
| GFP localization | Images of 131 protein with mitochondrial localzation assessed by GFP tagging and microscopy |
Download MitoCarta2.0 datasets
| Human.MitoCarta2.0.xls | Excel database containing detailed information on the 1158 mitochondrial human genes, as well as integrated scores of mitochondrial localization for all ~20,000 human genes with well-defined mouse homologs from HomoloGene | |
| Mouse.MitoCarta2.0.xls | Excel database containing detailed information on the 1158 mitochondrial mouse genes, as well as integrated scores of mitochondrial localization for all ~20,000 mouse genes | |
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HumanMitoCarta2.0.sql Human.MitoCarta2.0.txt |
MySQL database containing detailed information on the 1158 mitochondrial human genes, as well as integrated scores of mitochondrial localization for all ~20,000 human genes | |
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Mouse.MitoCarta2.0.sql Mouse.MitoCarta2.0.txt |
MySQL database containing detailed information on the 1158 mitochondrial mouse genes, as well as integrated scores of mitochondrial localization for all ~20,000 mouse genes | |
| Human.MitoCarta2.0.bed |
BED file of human MitoCarta2.0 genes within UCSC assembly hg19. Use UCSC's Add Custom Tracks to add URL http://www.broadinstitute.org/ftp/ distribution/metabolic/ papers/Pagliarini/MitoCarta2.0/ |
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| Mouse.MitoCarta2.0.bed |
BED file of mouse MitoCarta2.0 genes within UCSC assembly mm10. Use UCSC's Add Custom Tracks to add URL http://www.broadinstitute.org/ftp/ distribution/metabolic/ papers/Pagliarini/MitoCarta2.0/ |
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| Human.MitoCarta2.0.fasta | Fasta file of human MitoCarta2.0 RefSeq protein sequences | |
| Mouse.MitoCarta2.0.fasta | Fasta file of mouse MitoCarta2.0 RefSeq protein sequences | |
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Excel file of 92366 unique MS/MS peptides detected in 14 mouse tissues |
