Probing enzymatic activity inside living cells using a nanowire-cell "sandwich" assay.
Authors | |
Keywords | |
Abstract | Developing a detailed understanding of enzyme function in the context of an intracellular signal transduction pathway requires minimally invasive methods for probing enzyme activity in situ. Here, we describe a new method for monitoring enzyme activity in living cells by sandwiching live cells between two vertical silicon nanowire (NW) arrays. Specifically, we use the first NW array to immobilize the cells and then present enzymatic substrates intracellularly via the second NW array by utilizing the NWs' ability to penetrate cellular membranes without affecting cells' viability or function. This strategy, when coupled with fluorescence microscopy and mass spectrometry, enables intracellular examination of protease, phosphatase, and protein kinase activities, demonstrating the assay's potential in uncovering the physiological roles of various enzymes. |
Year of Publication | 2013
|
Journal | Nano Lett
|
Volume | 13
|
Issue | 1
|
Pages | 153-8
|
Date Published | 2013 Jan 09
|
ISSN | 1530-6992
|
DOI | 10.1021/nl3037068
|
PubMed ID | 23244056
|
PubMed Central ID | PMC3541459
|
Links | |
Grant list | 5DP1OD003893-03 / OD / NIH HHS / United States
P50 HG006193 / HG / NHGRI NIH HHS / United States
DP1 DA035083 / DA / NIDA NIH HHS / United States
DP1 OD003893 / OD / NIH HHS / United States
1P50HG006193-01 / HG / NHGRI NIH HHS / United States
|