|Publication Type||Journal Article|
|Year of Publication||2003|
|Authors||Lamb, J, Ramaswamy, S, Ford, HL, Contreras, B, Martinez, RV, Kittrell, FS, Zahnow, CA, Patterson, N, Golub, TR, Ewen, ME|
|Pages||323 - 34|
|Keywords||Cancer, CCAAT-Enhancer-Binding Protein-beta, Cyclin D1, Databases, Gene Expression Profiling, Gene Expression Regulation, Genetic, Humans, Neoplasm Proteins, Neoplasms, Neoplastic, Oligonucleotide Array Sequence Analysis, Promoter Regions (Genetics), Statistics|
Here we describe how patterns of gene expression in human tumors have been deconvoluted to reveal a mechanism of action for the cyclin D1 oncogene. Computational analysis of the expression patterns of thousands of genes across hundreds of tumor specimens suggested that a transcription factor, C/EBPbeta/Nf-Il6, participates in the consequences of cyclin D1 overexpression. Functional analyses confirmed the involvement of C/EBPbeta in the regulation of genes affected by cyclin D1 and established this protein as an indispensable effector of a potentially important facet of cyclin D1 biology. This work demonstrates that tumor gene expression databases can be used to study the function of a human oncogene in situ.