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Cell DOI:

Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria

Publication TypeJournal Article
Year of Publication2003
AuthorsMootha, VK, Bunkenborg, J, Olsen, JV, Hjerrild, M, Wisniewski, JR, Stahl, E, Bolouri, MS, Ray, HN, Sihag, S, Kamal, M, Patterson, N, Lander, ES, Mann, M
Pages629 - 40
Date Published2003/11/26/
ISBN Number0092-8674
KeywordsAnimals, Cancer, Cell Line, Cell Respiration, DNA, DNA Fingerprinting, Enzymologic, Gene Expression Regulation, Humans, Male, Messenger, Mice, Mitochondria, Mitochondrial, Mitochondrial Proteins, Oxidative Phosphorylation, Proteomics, RNA

Mitochondria are tailored to meet the metabolic and signaling needs of each cell. To explore its molecular composition, we performed a proteomic survey of mitochondria from mouse brain, heart, kidney, and liver and combined the results with existing gene annotations to produce a list of 591 mitochondrial proteins, including 163 proteins not previously associated with this organelle. The protein expression data were largely concordant with large-scale surveys of RNA abundance and both measures indicate tissue-specific differences in organelle composition. RNA expression profiles across tissues revealed networks of mitochondrial genes that share functional and regulatory mechanisms. We also determined a larger "neighborhood" of genes whose expression is closely correlated to the mitochondrial genes. The combined analysis identifies specific genes of biological interest, such as candidates for mtDNA repair enzymes, offers new insights into the biogenesis and ancestry of mammalian mitochondria, and provides a framework for understanding the organelle's contribution to human disease.