Zebrafish behavioral profiling identifies multitarget antipsychotic-like compounds.
Authors | |
Abstract | Many psychiatric drugs act on multiple targets and therefore require screening assays that encompass a wide target space. With sufficiently rich phenotyping and a large sampling of compounds, it should be possible to identify compounds with desired mechanisms of action on the basis of behavioral profiles alone. Although zebrafish (Danio rerio) behavior has been used to rapidly identify neuroactive compounds, it is not clear what types of behavioral assays would be necessary to identify multitarget compounds such as antipsychotics. Here we developed a battery of behavioral assays in larval zebrafish to determine whether behavioral profiles can provide sufficient phenotypic resolution to identify and classify psychiatric drugs. Using the antipsychotic drug haloperidol as a test case, we found that behavioral profiles of haloperidol-treated zebrafish could be used to identify previously uncharacterized compounds with desired antipsychotic-like activities and multitarget mechanisms of action. |
Year of Publication | 2016
|
Journal | Nat Chem Biol
|
Volume | 12
|
Issue | 7
|
Pages | 559-66
|
Date Published | 2016 Jul
|
ISSN | 1552-4469
|
URL | |
DOI | 10.1038/nchembio.2097
|
PubMed ID | 27239787
|
PubMed Central ID | PMC4912417
|
Links | |
Grant list | R21 MH085205 / MH / NIMH NIH HHS / United States
T32 GM008284 / GM / NIGMS NIH HHS / United States
K01 MH091449 / MH / NIMH NIH HHS / United States
R44 GM093456 / GM / NIGMS NIH HHS / United States
T32 EB009383 / EB / NIBIB NIH HHS / United States
U01 MH105027 / MH / NIMH NIH HHS / United States
T32 HL007208 / HL / NHLBI NIH HHS / United States
R01 MH086867 / MH / NIMH NIH HHS / United States
R01 AA022583 / AA / NIAAA NIH HHS / United States
|