Transcriptome-scale RNase-footprinting of RNA-protein complexes.
Nat Biotechnol
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Abstract | Ribosome profiling is widely used to study translation in vivo, but not all sequence reads correspond to ribosome-protected RNA. Here we describe Rfoot, a computational pipeline that analyzes ribosomal profiling data and identifies native, nonribosomal RNA-protein complexes. We use Rfoot to precisely map RNase-protected regions within small nucleolar RNAs, spliceosomal RNAs, microRNAs, tRNAs, long noncoding (lnc)RNAs and 3' untranslated regions of mRNAs in human cells. We show that RNAs of the same class can show differential complex association. Although only a subset of lncRNAs show RNase footprints, many of these have multiple footprints, and the protected regions are evolutionarily conserved, suggestive of biological functions. |
Year of Publication | 2016
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Journal | Nat Biotechnol
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Volume | 34
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Issue | 4
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Pages | 410-3
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Date Published | 2016 Apr
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ISSN | 1546-1696
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URL | |
DOI | 10.1038/nbt.3441
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PubMed ID | 26900662
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PubMed Central ID | PMC4824641
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Grant list | Howard Hughes Medical Institute / United States
P50 HG006193 / HG / NHGRI NIH HHS / United States
R01 CA107486 / CA / NCI NIH HHS / United States
CA 107486 / CA / NCI NIH HHS / United States
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