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Methods Mol Biol DOI:10.1007/978-1-4939-3524-6_13

Using the CPTAC Assay Portal to Identify and Implement Highly Characterized Targeted Proteomics Assays.

Publication TypeJournal Article
Year of Publication2016
AuthorsWhiteaker, JR, Halusa, GN, Hoofnagle, AN, Sharma, V, MacLean, B, Yan, P, Wrobel, JA, Kennedy, J, Mani, DR, Zimmerman, LJ, Meyer, MR, Mesri, M, Boja, E, Carr, SA, Chan, DW, Chen, X, Chen, J, Davies, SR, Ellis, MJC, Fenyö, D, Hiltke, T, Ketchum, KA, Kinsinger, C, Kuhn, E, Liebler, DC, Liu, T, Loss, M, MacCoss, MJ, Qian, W-J, Rivers, R, Rodland, KD, Ruggles, KV, Scott, MG, Smith, RD, Thomas, S, R Townsend, R, Whiteley, G, Wu, C, Zhang, H, Zhang, Z, Rodriguez, H, Paulovich, AG
JournalMethods Mol Biol
Volume1410
Pages223-36
Date Published2016
ISSN1940-6029
KeywordsHumans, Mass Spectrometry, Peptides, Protein Processing, Post-Translational, Proteins, Proteomics
Abstract

The Clinical Proteomic Tumor Analysis Consortium (CPTAC) of the National Cancer Institute (NCI) has launched an Assay Portal (http://assays.cancer.gov) to serve as an open-source repository of well-characterized targeted proteomic assays. The portal is designed to curate and disseminate highly characterized, targeted mass spectrometry (MS)-based assays by providing detailed assay performance characterization data, standard operating procedures, and access to reagents. Assay content is accessed via the portal through queries to find assays targeting proteins associated with specific cellular pathways, protein complexes, or specific chromosomal regions. The position of the peptide analytes for which there are available assays are mapped relative to other features of interest in the protein, such as sequence domains, isoforms, single nucleotide polymorphisms, and posttranslational modifications. The overarching goals are to enable robust quantification of all human proteins and to standardize the quantification of targeted MS-based assays to ultimately enable harmonization of results over time and across laboratories.

URLhttp://dx.doi.org/10.1007/978-1-4939-3524-6_13
DOI10.1007/978-1-4939-3524-6_13
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/26867747?dopt=Abstract

Alternate JournalMethods Mol. Biol.
PubMed ID26867747
PubMed Central IDPMC5017244
Grant ListU24 CA160034 / CA / NCI NIH HHS / United States
U24 CA159988 / CA / NCI NIH HHS / United States
U01 CA164186 / CA / NCI NIH HHS / United States
U24CA159988 / CA / NCI NIH HHS / United States
R01 GM103551 / GM / NIGMS NIH HHS / United States
U24CA160036 / CA / NCI NIH HHS / United States
U24 CA160036 / CA / NCI NIH HHS / United States
U24 CA160019 / CA / NCI NIH HHS / United States
U01CA164186 / CA / NCI NIH HHS / United States
U24 CA160035 / CA / NCI NIH HHS / United States
U24CA160034 / CA / NCI NIH HHS / United States
U24CA160035 / CA / NCI NIH HHS / United States
U24CA160019 / CA / NCI NIH HHS / United States