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Cell Rep DOI:10.1016/j.celrep.2015.12.051

A Genome-wide RNAi Screen for Microtubule Bundle Formation and Lysosome Motility Regulation in Drosophila S2 Cells.

Publication TypeJournal Article
Year of Publication2016
AuthorsJolly, AL, Luan, C-H, Dusel, BE, Dunne, SF, Winding, M, Dixit, VJ, Robins, C, Saluk, JL, Logan, DJ, Carpenter, AE, Sharma, M, Dean, D, Cohen, AR, Gelfand, VI
JournalCell Rep
Date Published2016 Jan 26
KeywordsAnimals, Bayes Theorem, Cells, Cultured, Drosophila, Drosophila Proteins, Dyneins, Genome, Lysosomes, Microtubules, Phenotype, rab GTP-Binding Proteins, RNA Interference, RNA, Double-Stranded, Time-Lapse Imaging

Long-distance intracellular transport of organelles, mRNA, and proteins ("cargo") occurs along the microtubule cytoskeleton by the action of kinesin and dynein motor proteins, but the vast network of factors involved in regulating intracellular cargo transport are still unknown. We capitalize on the Drosophila melanogaster S2 model cell system to monitor lysosome transport along microtubule bundles, which require enzymatically active kinesin-1 motor protein for their formation. We use an automated tracking program and a naive Bayesian classifier for the multivariate motility data to analyze 15,683 gene phenotypes and find 98 proteins involved in regulating lysosome motility along microtubules and 48 involved in the formation of microtubule filled processes in S2 cells. We identify innate immunity genes, ion channels, and signaling proteins having a role in lysosome motility regulation and find an unexpected relationship between the dynein motor, Rab7a, and lysosome motility regulation.


Alternate JournalCell Rep
PubMed ID26774481
PubMed Central IDPMC4868501
Grant ListP30 CA060553 / CA / NCI NIH HHS / United States
R01 GM052111 / GM / NIGMS NIH HHS / United States
R01 GM089652 / GM / NIGMS NIH HHS / United States