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G3 (Bethesda) DOI:10.1534/g3.117.043547

Whole Genome Sequence of the Heterozygous Clinical Isolate 81-B-5.

Publication TypeJournal Article
Year of Publication2017
AuthorsCuomo, CA, Shea, T, Yang, B, Rao, R, Forche, A
JournalG3 (Bethesda)
Date Published2017 09 07
KeywordsCandida, Candidiasis, Chromosomes, Fungal, Computational Biology, Genes, Mating Type, Fungal, Genetic Association Studies, Genetic Variation, Genome, Fungal, Genomics, Heterozygote, Humans, Karyotype, Loss of Heterozygosity, Molecular Sequence Annotation, Phenotype, Phylogeny, Polymorphism, Single Nucleotide, Sequence Analysis, DNA, Whole Genome Sequencing

is a diploid, heterozygous yeast that is an opportunistic fungal pathogen in immunocompromised patients. This species also is utilized for fermenting cocoa beans during chocolate production. One major concern in the clinical setting is the innate resistance of this species to the most commonly used antifungal drug fluconazole. Here, we report a high-quality genome sequence and assembly for the first clinical isolate of , strain 81-B-5, into 11 scaffolds generated with PacBio sequencing technology. Gene annotation and comparative analysis revealed a unique profile of transporters that could play a role in drug resistance or adaptation to different environments. In addition, we show that, while 82% of the genome is highly heterozygous, a 2.0 Mb region of the largest scaffold has undergone loss of heterozygosity. This genome will serve as a reference for further genetic studies of this pathogen.


Alternate JournalG3 (Bethesda)
PubMed ID28696923
PubMed Central IDPMC5592916
Grant ListP20 GM103423 / GM / NIGMS NIH HHS / United States
R15 AI090633 / AI / NIAID NIH HHS / United States
U19 AI110818 / AI / NIAID NIH HHS / United States