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G3 (Bethesda) DOI:10.1534/g3.117.043547

Whole Genome Sequence of the Heterozygous Clinical Isolate Candida krusei 81-B-5.

Publication TypeJournal Article
Year of Publication2017
AuthorsCuomo, CA, Shea, T, Yang, B, Rao, R, Forche, A
JournalG3 (Bethesda)
Date Published2017 Jul 10
ISSN2160-1836
Abstract

Candida krusei is a diploid, heterozygous yeast that is an opportunistic fungal pathogen in immunocompromised patients. This species also is utilized for fermenting cocoa beans during chocolate production. One major concern in the clinical setting is the innate resistance of this species to the most commonly used antifungal drug fluconazole. Here we report a high-quality genome sequence and assembly for the first clinical isolate of C. krusei, strain 81-B-5, into 11 scaffolds generated with PacBio sequencing technology. Gene annotation and comparative analysis revealed a unique profile of transporters that could play a role in drug resistance or adaptation to different environments. In addition, we show that while 82% of the genome is highly heterozygous, a 2.0 Mb region of the largest scaffold has undergone loss of heterozygosity. This genome will serve as a reference for further genetic studies of this pathogen.

DOI10.1534/g3.117.043547
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/28696923?dopt=Abstract

Alternate JournalG3 (Bethesda)
PubMed ID28696923
Grant ListU19 AI110818 / AI / NIAID NIH HHS / United States