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PLoS One DOI:10.1371/journal.pone.0046984

The success of acinetobacter species; genetic, metabolic and virulence attributes.

Publication TypeJournal Article
Year of Publication2012
AuthorsPeleg, AY, de Breij, A, Adams, MD, Cerqueira, GM, Mocali, S, Galardini, M, Nibbering, PH, Earl, AM, Ward, DV, Paterson, DL, Seifert, H, Dijkshoorn, L
JournalPLoS One
Volume7
Issue10
Pagese46984
Date Published2012
ISSN1932-6203
KeywordsAcinetobacter, Acinetobacter baumannii, Acinetobacter calcoaceticus, Acinetobacter Infections, Animals, Bacterial Proteins, Genes, Bacterial, Genome, Bacterial, Humans, Mice, Operon, Siderophores, Skin, Species Specificity, Virulence
Abstract

An understanding of why certain Acinetobacter species are more successful in causing nosocomial infections, transmission and epidemic spread in healthcare institutions compared with other species is lacking. We used genomic, phenotypic and virulence studies to identify differences between Acinetobacter species. Fourteen strains representing nine species were examined. Genomic analysis of six strains showed that the A. baumannii core genome contains many genes important for diverse metabolism and survival in the host. Most of the A. baumannii core genes were also present in one or more of the less clinically successful species. In contrast, when the accessory genome of an individual A. baumannii strain was compared to a strain of a less successful species (A. calcoaceticus RUH2202), many operons with putative virulence function were found to be present only in the A. baumannii strain, including the csu operon, the acinetobactin chromosomal cluster, and bacterial defence mechanisms. Phenotype microarray analysis showed that compared to A. calcoaceticus (RUH2202), A. baumannii ATCC 19606(T) was able to utilise nitrogen sources more effectively and was more tolerant to pH, osmotic and antimicrobial stress. Virulence differences were also observed, with A. baumannii ATCC 19606(T), A. pittii SH024, and A. nosocomialis RUH2624 persisting and forming larger biofilms on human skin than A. calcoaceticus. A. baumannii ATCC 19606(T) and A. pittii SH024 were also able to survive in a murine thigh infection model, whereas the other two species were eradicated. The current study provides important insights into the elucidation of differences in clinical relevance among Acinetobacter species.

URLhttp://dx.plos.org/10.1371/journal.pone.0046984
DOI10.1371/journal.pone.0046984
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/23144699?dopt=Abstract

Alternate JournalPLoS ONE
PubMed ID23144699
PubMed Central IDPMC3483291
Grant ListR01 GM094403 / GM / NIGMS NIH HHS / United States
/ / Medical Research Council / United Kingdom