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Eukaryot Cell DOI:10.1128/EC.00249-12

Identification of the mating-type (MAT) locus that controls sexual reproduction of Blastomyces dermatitidis.

Publication TypeJournal Article
Year of Publication2013
AuthorsLi, W, Sullivan, TD, Walton, E, Averette, AFloyd, Sakthikumar, S, Cuomo, CA, Klein, BS, Heitman, J
JournalEukaryot Cell
Volume12
Issue1
Pages109-17
Date Published2013 Jan
ISSN1535-9786
KeywordsBlastomyces, DNA Transposable Elements, Evolution, Molecular, Genes, Mating Type, Fungal, Genome, Fungal, Hyphae, Models, Genetic, Recombination, Genetic, Reproduction, Sequence Analysis, DNA
Abstract

Blastomyces dermatitidis is a dimorphic fungal pathogen that primarily causes blastomycosis in the midwestern and northern United States and Canada. While the genes controlling sexual development have been known for a long time, the genes controlling sexual reproduction of B. dermatitidis (teleomorph, Ajellomyces dermatitidis) are unknown. We identified the mating-type (MAT) locus in the B. dermatitidis genome by comparative genomic approaches. The B. dermatitidis MAT locus resembles those of other dimorphic fungi, containing either an alpha-box (MAT1-1) or an HMG domain (MAT1-2) gene linked to the APN2, SLA2, and COX13 genes. However, in some strains of B. dermatitidis, the MAT locus harbors transposable elements (TEs) that make it unusually large compared to the MAT locus of other dimorphic fungi. Based on the MAT locus sequences of B. dermatitidis, we designed specific primers for PCR determination of the mating type. Two B. dermatitidis isolates of opposite mating types were cocultured on mating medium. Immature sexual structures were observed starting at 3 weeks of coculture, with coiled-hyphae-containing cleistothecia developing over the next 3 to 6 weeks. Genetic recombination was detected in potential progeny by mating-type determination, PCR-restriction fragment length polymorphism (PCR-RFLP), and random amplification of polymorphic DNA (RAPD) analyses, suggesting that a meiotic sexual cycle might have been completed. The F1 progeny were sexually fertile when tested with strains of the opposite mating type. Our studies provide a model for the evolution of the MAT locus in the dimorphic and closely related fungi and open the door to classic genetic analysis and studies on the possible roles of mating and mating type in infection and virulence.

URLhttp://ec.asm.org/cgi/pmidlookup?view=long&pmid=23143684
DOI10.1128/EC.00249-12
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/23143684?dopt=Abstract

Alternate JournalEukaryotic Cell
PubMed ID23143684
PubMed Central IDPMC3535845
Grant ListR37 AI039115 / AI / NIAID NIH HHS / United States
AI35681 / AI / NIAID NIH HHS / United States
R37 AI035681 / AI / NIAID NIH HHS / United States
HHSN272200900018C / AI / NIAID NIH HHS / United States
R01 AI039115 / AI / NIAID NIH HHS / United States
R37 AI39115 / AI / NIAID NIH HHS / United States
R01 AI035681 / AI / NIAID NIH HHS / United States
HHSN272200900018C / / PHS HHS / United States