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PLoS One DOI:10.1371/journal.pone.0039315

Evaluation of 16S rDNA-based community profiling for human microbiome research.

Publication TypeJournal Article
Year of Publication2012
Corporate AuthorsJumpstart Consortium Human Microbiome Project Data Generation Working Group
JournalPLoS One
Volume7
Issue6
Pagese39315
Date Published2012
ISSN1932-6203
KeywordsBacteria, Bayes Theorem, Humans, Metagenome, RNA, Ribosomal, 16S
Abstract

The Human Microbiome Project will establish a reference data set for analysis of the microbiome of healthy adults by surveying multiple body sites from 300 people and generating data from over 12,000 samples. To characterize these samples, the participating sequencing centers evaluated and adopted 16S rDNA community profiling protocols for ABI 3730 and 454 FLX Titanium sequencing. In the course of establishing protocols, we examined the performance and error characteristics of each technology, and the relationship of sequence error to the utility of 16S rDNA regions for classification- and OTU-based analysis of community structure. The data production protocols used for this work are those used by the participating centers to produce 16S rDNA sequence for the Human Microbiome Project. Thus, these results can be informative for interpreting the large body of clinical 16S rDNA data produced for this project.

URLhttp://dx.plos.org/10.1371/journal.pone.0039315
DOI10.1371/journal.pone.0039315
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/22720093?dopt=Abstract

Alternate JournalPLoS ONE
PubMed ID22720093
PubMed Central IDPMC3374619
Grant ListU54 HG004973 / HG / NHGRI NIH HHS / United States
U54HG004969 / HG / NHGRI NIH HHS / United States
HHSN272200900018C / AI / NIAID NIH HHS / United States
HHSN272200900008C / AI / NIAID NIH HHS / United States
HHSN272200900001C / AI / NIAID NIH HHS / United States
U54 AI084844 / AI / NIAID NIH HHS / United States
1U01HG004866 / HG / NHGRI NIH HHS / United States
U54 HG003273 / HG / NHGRI NIH HHS / United States
U01 HG004866 / HG / NHGRI NIH HHS / United States
U54AI084844 / AI / NIAID NIH HHS / United States
U54 HG004968 / HG / NHGRI NIH HHS / United States
U54HG004973 / HG / NHGRI NIH HHS / United States
U54HG003079 / HG / NHGRI NIH HHS / United States
HHSN2722009000018C / / PHS HHS / United States
U54 HG003079 / HG / NHGRI NIH HHS / United States
U54 HG004969 / HG / NHGRI NIH HHS / United States
U54HG004968 / HG / NHGRI NIH HHS / United States