Time-Resolved Analysis Reveals Rapid Dynamics and Broad Scope of the CBP/p300 Acetylome.

Cell
Authors
Brian Weinert
Takeo Narita
Shankha Satpathy
Balaji Srinivasan
Bogi Hansen
Christian Schölz
William Hamilton
Beth Zucconi
Wesley Wang
Wenshe Liu
Joshua Brickman
Edward Kesicki
Albert Lai
Kenneth Bromberg
Philip Cole
Chunaram Choudhary
Keywords
Animals
Humans
Mass Spectrometry
Mice
Cell Line
Peptides
Signal Transduction
Transcriptome
Acetylation
Histones
p300-CBP Transcription Factors
Gene Knockout Techniques
Acetyltransferases
Small Molecule Libraries
Recombinant Proteins
Isotope Labeling
Kinetics
Heterocyclic Compounds, 4 or More Rings
Half-Life
Receptors, Aryl Hydrocarbon
Abstract

The acetyltransferases CBP and p300 are multifunctional transcriptional co-activators. Here, we combined quantitative proteomics with CBP/p300-specific catalytic inhibitors, bromodomain inhibitor, and gene knockout to reveal a comprehensive map of regulated acetylation sites and their dynamic turnover rates. CBP/p300 acetylates thousands of sites, including signature histone sites and a multitude of sites on signaling effectors and enhancer-associated transcriptional regulators. Time-resolved acetylome analyses identified a subset of CBP/p300-regulated sites with very rapid (

Year of Publication
2018
Journal
Cell
Volume
174
Issue
1
Pages
231-244.e12
Date Published
2018 06 28
ISSN
1097-4172
DOI
10.1016/j.cell.2018.04.033
PubMed ID
29804834
PubMed Central ID
PMC6078418
Links
Grant list
K99 GM124357 / GM / NIGMS NIH HHS / United States
R37 GM062437 / GM / NIGMS NIH HHS / United States

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