Cas9 gRNA engineering for genome editing, activation and repression.

Nat Methods
Authors
Keywords
Abstract

We demonstrate that by altering the length of Cas9-associated guide RNA (gRNA) we were able to control Cas9 nuclease activity and simultaneously perform genome editing and transcriptional regulation with a single Cas9 protein. We exploited these principles to engineer mammalian synthetic circuits with combined transcriptional regulation and kill functions governed by a single multifunctional Cas9 protein.

Year of Publication
2015
Journal
Nat Methods
Volume
12
Issue
11
Pages
1051-4
Date Published
2015 Nov
ISSN
1548-7105
URL
DOI
10.1038/nmeth.3580
PubMed ID
26344044
PubMed Central ID
PMC4666719
Links
Grant list
P50 HG005550 / HG / NHGRI NIH HHS / United States
R01 CA155320 / CA / NCI NIH HHS / United States
T32 GM007598 / GM / NIGMS NIH HHS / United States
P50 GM098792 / GM / NIGMS NIH HHS / United States
Canadian Institutes of Health Research / Canada
T32 CA009216 / CA / NCI NIH HHS / United States
5T32CA009216-34 / CA / NCI NIH HHS / United States
5R01CA155320-04 / CA / NCI NIH HHS / United States