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Cell DOI:10.1016/j.cell.2015.07.020

Clinical Sequencing Uncovers Origins and Evolution of Lassa Virus.

Publication TypeJournal Article
Year of Publication2015
AuthorsAndersen, KG, B Shapiro, J, Matranga, CB, Sealfon, R, Lin, AE, Moses, LM, Folarin, OA, Goba, A, Odia, I, Ehiane, PE, Momoh, M, England, EM, Winnicki, S, Branco, LM, Gire, SK, Phelan, E, Tariyal, R, Tewhey, R, Omoniwa, O, Fullah, M, Fonnie, R, Fonnie, M, Kanneh, L, Jalloh, S, Gbakie, M, Saffa, S, Karbo, K, Gladden, AD, Qu, J, Stremlau, M, Nekoui, M, Finucane, HK, Tabrizi, S, Vitti, JJ, Birren, B, Fitzgerald, M, McCowan, C, Ireland, A, Berlin, AM, Bochicchio, J, Tazon-Vega, B, Lennon, NJ, Ryan, EM, Bjornson, Z, Milner, DA, Lukens, AK, Broodie, N, Rowland, M, Heinrich, M, Akdag, M, Schieffelin, JS, Levy, D, Akpan, H, Bausch, DG, Rubins, K, McCormick, JB, Lander, ES, Günther, S, Hensley, L, Okogbenin, S, Schaffner, SF, Okokhere, PO, S Khan, H, Grant, DS, Akpede, GO, Asogun, DA, Gnirke, A, Levin, JZ, Happi, CT, Garry, RF, Sabeti, PC
Corporate AuthorsViral Hemorrhagic Fever Consortium
Date Published2015 Aug 13
KeywordsAfrica, Western, Animals, Biological Evolution, Disease Reservoirs, Ebolavirus, Genetic Variation, Genome, Viral, Glycoproteins, Hemorrhagic Fever, Ebola, Humans, Lassa Fever, Lassa virus, Murinae, Mutation, Nigeria, RNA, Viral, Viral Proteins, Zoonoses

The 2013-2015 West African epidemic of Ebola virus disease (EVD) reminds us of how little is known about biosafety level 4 viruses. Like Ebola virus, Lassa virus (LASV) can cause hemorrhagic fever with high case fatality rates. We generated a genomic catalog of almost 200 LASV sequences from clinical and rodent reservoir samples. We show that whereas the 2013-2015 EVD epidemic is fueled by human-to-human transmissions, LASV infections mainly result from reservoir-to-human infections. We elucidated the spread of LASV across West Africa and show that this migration was accompanied by changes in LASV genome abundance, fatality rates, codon adaptation, and translational efficiency. By investigating intrahost evolution, we found that mutations accumulate in epitopes of viral surface proteins, suggesting selection for immune escape. This catalog will serve as a foundation for the development of vaccines and diagnostics. VIDEO ABSTRACT.


Alternate JournalCell
PubMed ID26276630
PubMed Central IDPMC4537774
Grant ListR01 AI114855 / AI / NIAID NIH HHS / United States
HHSN272201000022C / / PHS HHS / United States
HHSN272200900018C / AI / NIAID NIH HHS / United States
1DP2OD006514-01 / OD / NIH HHS / United States
U19 AI109762 / AI / NIAID NIH HHS / United States
K99 HG008179 / HG / NHGRI NIH HHS / United States
HHSN272200900018C / / PHS HHS / United States
HHSN272201000022C / AI / NIAID NIH HHS / United States
U19AI110818 / AI / NIAID NIH HHS / United States
HHSN272200900049C / AI / NIAID NIH HHS / United States
DP2 OD006514 / OD / NIH HHS / United States
U19 AI110818 / AI / NIAID NIH HHS / United States
HHSN272200900049C / / PHS HHS / United States