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Nat Methods DOI:10.1038/nmeth.3507

Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells.

Publication TypeJournal Article
Year of Publication2015
AuthorsPaull, D, Sevilla, A, Zhou, H, Hahn, AKim, Kim, H, Napolitano, C, Tsankov, A, Shang, L, Krumholz, K, Jagadeesan, P, Woodard, CM, Sun, B, Vilboux, T, Zimmer, M, Forero, E, Moroziewicz, DN, Martinez, H, Malicdan, MChristine, Weiss, KA, Vensand, LB, Dusenberry, CR, Polus, H, Sy, KTherese L, Kahler, DJ, Gahl, WA, Solomon, SL, Chang, S, Meissner, A, Eggan, K, Noggle, SA
JournalNat Methods
Date Published2015 Sep
KeywordsBatch Cell Culture Techniques, Cell Differentiation, Cell Separation, Cells, Cultured, Equipment Design, Equipment Failure Analysis, Fibroblasts, Humans, Induced Pluripotent Stem Cells, Microfluidic Analytical Techniques, Robotics

Induced pluripotent stem cells (iPSCs) are an essential tool for modeling how causal genetic variants impact cellular function in disease, as well as an emerging source of tissue for regenerative medicine. The preparation of somatic cells, their reprogramming and the subsequent verification of iPSC pluripotency are laborious, manual processes limiting the scale and reproducibility of this technology. Here we describe a modular, robotic platform for iPSC reprogramming enabling automated, high-throughput conversion of skin biopsies into iPSCs and differentiated cells with minimal manual intervention. We demonstrate that automated reprogramming and the pooled selection of polyclonal pluripotent cells results in high-quality, stable iPSCs. These lines display less line-to-line variation than either manually produced lines or lines produced through automation followed by single-colony subcloning. The robotic platform we describe will enable the application of iPSCs to population-scale biomedical problems including the study of complex genetic diseases and the development of personalized medicines.


Alternate JournalNat. Methods
PubMed ID26237226
Grant ListP01GM099117 / GM / NIGMS NIH HHS / United States