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Elife DOI:10.7554/eLife.06974

Chromerid genomes reveal the evolutionary path from photosynthetic algae to obligate intracellular parasites.

Publication TypeJournal Article
Year of Publication2015
AuthorsWoo, YH, Ansari, H, Otto, TD, Klinger, CM, Kolisko, M, Michálek, J, Saxena, A, Shanmugam, D, Tayyrov, A, Veluchamy, A, Ali, S, Bernal, A, del Campo, J, Cihlář, J, Flegontov, P, Gornik, SG, Hajdušková, E, Horák, A, Janouškovec, J, Katris, NJ, Mast, FD, Miranda-Saavedra, D, Mourier, T, Naeem, R, Nair, M, Panigrahi, AK, Rawlings, ND, Padron-Regalado, E, Ramaprasad, A, Samad, N, Tomčala, A, Wilkes, J, Neafsey, DE, Doerig, C, Bowler, C, Keeling, PJ, Roos, DS, Dacks, JB, Templeton, TJ, Waller, RF, Lukeš, J, Oborník, M, Pain, A
JournalElife
Volume4
Pagese06974
Date Published2015 Jul 15
ISSN2050-084X
KeywordsAlveolata, DNA, Algal, Evolution, Molecular, Gene Expression Profiling, Molecular Sequence Data, Sequence Analysis, DNA
Abstract

The eukaryotic phylum Apicomplexa encompasses thousands of obligate intracellular parasites of humans and animals with immense socio-economic and health impacts. We sequenced nuclear genomes of Chromera velia and Vitrella brassicaformis, free-living non-parasitic photosynthetic algae closely related to apicomplexans. Proteins from key metabolic pathways and from the endomembrane trafficking systems associated with a free-living lifestyle have been progressively and non-randomly lost during adaptation to parasitism. The free-living ancestor contained a broad repertoire of genes many of which were repurposed for parasitic processes, such as extracellular proteins, components of a motility apparatus, and DNA- and RNA-binding protein families. Based on transcriptome analyses across 36 environmental conditions, Chromera orthologs of apicomplexan invasion-related motility genes were co-regulated with genes encoding the flagellar apparatus, supporting the functional contribution of flagella to the evolution of invasion machinery. This study provides insights into how obligate parasites with diverse life strategies arose from a once free-living phototrophic marine alga.

URLhttp://dx.doi.org/10.7554/eLife.06974
DOI10.7554/eLife.06974
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/26175406?dopt=Abstract

Alternate JournalElife
PubMed ID26175406
PubMed Central IDPMC4501334
Grant ListHHSN272200900018C / / PHS HHS / United States