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Proteomics DOI:10.1002/pmic.201400142

Quantitative proteomics suggests decrease in the secretogranin-1 cerebrospinal fluid levels during the disease course of multiple sclerosis.

Publication TypeJournal Article
Year of Publication2015
AuthorsKroksveen, AC, Jaffe, JD, Aasebø, E, Barsnes, H, Bjørlykke, Y, Franciotta, D, Keshishian, H, Myhr, K-M, Opsahl, JA, van Pesch, V, Teunissen, CE, Torkildsen, Ø, Ulvik, RJ, Vethe, H, Carr, SA, Berven, FS
Date Published2015 Oct
KeywordsAdult, Biomarkers, Chromogranin B, Disease Progression, Down-Regulation, Female, Humans, Male, Mass Spectrometry, Middle Aged, Multiple Sclerosis, Proteomics

Multiple sclerosis (MS) is a chronic inflammatory disease of the CNS with unknown cause. Proteins with different abundance in the cerebrospinal fluid (CSF) from relapsing-remitting MS (RRMS) patients and neurological controls could give novel insight to the MS pathogenesis and be used to improve diagnosis, predict prognosis and disease course, and guide in therapy decisions. We combined iTRAQ labeling and Orbitrap mass spectrometry to discover proteins with different CSF abundance between six RRMS patients and 18 neurological disease controls. From 777 quantified proteins seven were selected as biomarker candidates, namely chitinase-3-like protein 1, secretogranin-1 (Sg1), cerebellin-1, neuroserpin, cell surface glycoprotein MUC18, testican-2 and glutamate receptor 4. An independent sample set of 13 early-MS patients, 13 RRMS patients and 13 neurological controls was used in a multiple reaction monitoring verification study. We found the intracellular calcium binding protein Sg1 to be increased in early-MS patients compared to RRMS and neurological controls. Sg1 should be included in further studies to elucidate its role in the early phases of MS pathogenesis and its potential as a biomarker for this disease.


Alternate JournalProteomics
PubMed ID26152395