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Nat Commun DOI:10.1038/ncomms8121

Contrasting host-pathogen interactions and genome evolution in two generalist and specialist microsporidian pathogens of mosquitoes.

Publication TypeJournal Article
Year of Publication2015
AuthorsDesjardins, CA, Sanscrainte, ND, Goldberg, JM, Heiman, D, Young, S, Zeng, Q, Madhani, HD, Becnel, JJ, Cuomo, CA
JournalNat Commun
Date Published2015 May 13
KeywordsAedes, Animals, Genome, Fungal, Host-Pathogen Interactions, Larva, Microsporidia, Polymorphism, Genetic, RNA, Fungal

Obligate intracellular pathogens depend on their host for growth yet must also evade detection by host defenses. Here we investigate host adaptation in two Microsporidia, the specialist Edhazardia aedis and the generalist Vavraia culicis, pathogens of disease vector mosquitoes. Genomic analysis and deep RNA-Seq across infection time courses reveal fundamental differences between these pathogens. E. aedis retains enhanced cell surface modification and signalling capacity, upregulating protein trafficking and secretion dynamically during infection. V. culicis is less dependent on its host for basic metabolites and retains a subset of spliceosomal components, with a transcriptome broadly focused on growth and replication. Transcriptional profiling of mosquito immune responses reveals that response to infection by E. aedis differs dramatically depending on the mode of infection, and that antimicrobial defensins may play a general role in mosquito defense against Microsporidia. This analysis illuminates fundamentally different evolutionary paths and host interplay of specialist and generalist pathogens.


Alternate JournalNat Commun
PubMed ID25968466
PubMed Central IDPMC4435813
Grant ListHHSN272200900018C / AI / NIAID NIH HHS / United States
U19 AI110818 / AI / NIAID NIH HHS / United States
HHSN272200900018C / / PHS HHS / United States