Rapid, deep and precise profiling of the plasma proteome with multi-nanoparticle protein corona.

Nat Commun
Authors
Keywords
Abstract

Large-scale, unbiased proteomics studies are constrained by the complexity of the plasma proteome. Here we report a highly parallel protein quantitation platform integrating nanoparticle (NP) protein coronas with liquid chromatography-mass spectrometry for efficient proteomic profiling. A protein corona is a protein layer adsorbed onto NPs upon contact with biofluids. Varying the physicochemical properties of engineered NPs translates to distinct protein corona patterns enabling differential and reproducible interrogation of biological samples, including deep sampling of the plasma proteome. Spike experiments confirm a linear signal response. The median coefficient of variation was 22%. We screened 43 NPs and selected a panel of 5, which detect more than 2,000 proteins from 141 plasma samples using a 96-well automated workflow in a pilot non-small cell lung cancer classification study. Our streamlined workflow combines depth of coverage and throughput with precise quantification based on unique interactions between proteins and NPs engineered for deep and scalable quantitative proteomic studies.

Year of Publication
2020
Journal
Nat Commun
Volume
11
Issue
1
Pages
3662
Date Published
2020 07 22
ISSN
2041-1723
DOI
10.1038/s41467-020-17033-7
PubMed ID
32699280
PubMed Central ID
PMC7376165
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