Highly efficient Cas9-mediated transcriptional programming.

Nat Methods
Authors
Keywords
Abstract

The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9. We demonstrate its utility in activating endogenous coding and noncoding genes, targeting several genes simultaneously and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs).

Year of Publication
2015
Journal
Nat Methods
Volume
12
Issue
4
Pages
326-8
Date Published
2015 Apr
ISSN
1548-7105
URL
DOI
10.1038/nmeth.3312
PubMed ID
25730490
PubMed Central ID
PMC4393883
Links
Grant list
P50 HG005550 / HG / NHGRI NIH HHS / United States
T32 GM007598 / GM / NIGMS NIH HHS / United States
R01 CA173712 / CA / NCI NIH HHS / United States
T32 CA009216 / CA / NCI NIH HHS / United States
5T32CA009216-34 / CA / NCI NIH HHS / United States