Highly efficient Cas9-mediated transcriptional programming.
Nat Methods
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Abstract | The RNA-guided nuclease Cas9 can be reengineered as a programmable transcription factor. However, modest levels of gene activation have limited potential applications. We describe an improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9. We demonstrate its utility in activating endogenous coding and noncoding genes, targeting several genes simultaneously and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs). |
Year of Publication | 2015
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Journal | Nat Methods
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Volume | 12
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Issue | 4
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Pages | 326-8
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Date Published | 2015 Apr
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ISSN | 1548-7105
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URL | |
DOI | 10.1038/nmeth.3312
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PubMed ID | 25730490
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PubMed Central ID | PMC4393883
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Grant list | P50 HG005550 / HG / NHGRI NIH HHS / United States
T32 GM007598 / GM / NIGMS NIH HHS / United States
R01 CA173712 / CA / NCI NIH HHS / United States
T32 CA009216 / CA / NCI NIH HHS / United States
5T32CA009216-34 / CA / NCI NIH HHS / United States
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