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Curr Protoc Mol Biol DOI:10.1002/0471142727.mb3002s98

Targeted metabolomics.

Publication TypeJournal Article
Year of Publication2012
AuthorsRoberts, LD, Souza, AL, Gerszten, RE, Clish, CB
JournalCurr Protoc Mol Biol
VolumeChapter 30
PagesUnit 30.2.1-24
Date Published2012 Apr
KeywordsAnimals, Chromatography, Liquid, Humans, Mass Spectrometry, Metabolome, Metabolomics, Plasma

The metabolome is the terminal downstream product of the genome and consists of the total complement of all the low-molecular-weight molecules (metabolites) in a cell, tissue, or organism. Metabolomics aims to measure a wide breadth of small molecules in the context of physiological stimuli or disease states. Metabolomics methodologies fall into two distinct groups: untargeted metabolomics, an intended comprehensive analysis of all the measurable analytes in a sample including chemical unknowns, and targeted metabolomics, the measurement of defined groups of chemically characterized and biochemically annotated metabolites. The methodologies considered in this unit focus on the processes of conducting targeted metabolomics experiments, and the advantages of this general approach are highlighted herein. This unit outlines procedures for extracting nitrogenous metabolites (including amino acids), lipids, and intermediary metabolites (including TCA cycle oxoacids) from blood plasma. Specifically, protocols are described for analyzing these metabolites using targeted metabolomics experiments based on liquid chromatography-mass spectrometry.


Alternate JournalCurr Protoc Mol Biol
PubMed ID22470063
PubMed Central IDPMC3334318
Grant ListN01-HC-25195 / HC / NHLBI NIH HHS / United States
R01-DK081572 / DK / NIDDK NIH HHS / United States
R01 DK081572 / DK / NIDDK NIH HHS / United States
R01 DK081572-02 / DK / NIDDK NIH HHS / United States
R01 HL098280 / HL / NHLBI NIH HHS / United States
R01 HL098280-03 / HL / NHLBI NIH HHS / United States
R01-HL098280 / HL / NHLBI NIH HHS / United States
R01-HL096738 / HL / NHLBI NIH HHS / United States