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Clin Chem DOI:10.1373/clinchem.2012.199133

Reproducibility of metabolomic profiles among men and women in 2 large cohort studies.

Publication TypeJournal Article
Year of Publication2013
AuthorsTownsend, MK, Clish, CB, Kraft, P, Wu, C, Souza, AL, Deik, AA, Tworoger, SS, Wolpin, BM
JournalClin Chem
Date Published2013 Nov
KeywordsAdult, Aged, Chromatography, Liquid, Female, Humans, Male, Metabolome, Middle Aged, Pilot Projects, Prospective Studies, Reproducibility of Results, Sex Factors, Tandem Mass Spectrometry

BACKGROUND: Rigorous studies are necessary to demonstrate suitability of metabolomics platforms to profile metabolites in archived plasma within epidemiologic studies of human disease, for which attenuation of effect estimates due to measurement error is a key concern.

METHODS: Using a liquid chromatography-tandem mass spectrometry platform, we quantified 257 metabolites from archived plasma to evaluate metabolite interassay reproducibility, reproducibility with delayed processing, and within-person reproducibility over time. Interassay reproducibility was assessed with CVs from 60 duplicate plasma samples donated by participants in the Nurses' Health Study and Health Professionals Follow-up Study, and 20 QC pool plasma replicates. Metabolite reproducibility over a 24- to 48-h processing delay (n = 48 samples) and within-person reproducibility over 1-2 years (n = 80 samples) were assessed using Spearman and intraclass correlation coefficients (ICCs).


CONCLUSIONS: For potential use in epidemiologic studies, the majority of plasma metabolites had low CVs and were reproducible over a 24-h processing delay and within individuals over 1-2 years. Certain metabolites, such as carbohydrates and purine/pyrimidine derivatives, may be challenging to evaluate if samples have delayed processing.


Alternate JournalClin. Chem.
PubMed ID23897902
PubMed Central IDPMC3812240
Grant ListP01 CA087969 / CA / NCI NIH HHS / United States
P01 CA055075 / CA / NCI NIH HHS / United States
K07-CA140790 / CA / NCI NIH HHS / United States
/ / Howard Hughes Medical Institute / United States
R01-CA49449 / CA / NCI NIH HHS / United States
K07 CA140790 / CA / NCI NIH HHS / United States
P01-CA87969 / CA / NCI NIH HHS / United States
R01 CA049449 / CA / NCI NIH HHS / United States
P01-CA055075 / CA / NCI NIH HHS / United States