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Chem Biol DOI:10.1016/j.chembiol.2014.11.018

CDy6, a photostable probe for long-term real-time visualization of mitosis and proliferating cells.

Publication TypeJournal Article
Year of Publication2015
AuthorsJeong, Y-M, Duanting, Z, Hennig, H, Samanta, A, Agrawalla, BKeshari, Bray, M-A, Carpenter, AE, Chang, Y-T
JournalChem Biol
Date Published2015 Feb 19
KeywordsAnimals, Boron Compounds, Cell Line, Cell Proliferation, Fluorescent Dyes, HeLa Cells, Humans, Lysosomes, Mice, Mice, Inbred BALB C, Mice, Nude, Microscopy, Fluorescence, Mitosis, Time-Lapse Imaging, Transplantation, Heterologous

Long-term real-time visualization of lysosomal dynamics has been challenging at the onset of mitosis due to the lack of fluorescent probes enabling convenient imaging of dividing cells. We developed a long-term real-time photostable mitotic or proliferating marker, CDy6, a BODIPY-derived compound of designation yellow 6, which labels lysosome. In long-term real-time, CDy6 displayed a sharp increase in intensity and change in localization in mitosis, improved photostability, and decreased toxicity compared with other widely used lysosomal and DNA markers, and the ability to label cells in mouse xenograft models. Therefore, CDy6 may open new possibilities to target and trace lysosomal contents during mitosis and to monitor cell proliferation, which can further our knowledge of the basic underlying biological mechanisms in the management of cancer.


Alternate JournalChem. Biol.
PubMed ID25601075