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Neuron DOI:10.1016/j.neuron.2014.12.024

DeCoN: genome-wide analysis of in vivo transcriptional dynamics during pyramidal neuron fate selection in neocortex.

Publication TypeJournal Article
Year of Publication2015
AuthorsMolyneaux, BJ, Goff, LA, Brettler, AC, Chen, H-H, Hrvatin, S, Rinn, JL, Arlotta, P
JournalNeuron
Volume85
Issue2
Pages275-288
Date Published2015 Jan 21
ISSN1097-4199
KeywordsAnimals, Base Sequence, Cell Differentiation, Corpus Callosum, Flow Cytometry, Gene Expression Profiling, Gene Expression Regulation, Developmental, Matrix Attachment Region Binding Proteins, Mice, Molecular Sequence Data, Motor Neurons, Neocortex, Neurogenesis, Neurons, Pyramidal Cells, Pyramidal Tracts, Repressor Proteins, RNA, Long Noncoding, RNA, Messenger, Transcription Factors, Transcriptome, Tumor Suppressor Proteins
Abstract

UNLABELLED: Neuronal development requires a complex choreography of transcriptional decisions to obtain specific cellular identities. Realizing the ultimate goal of identifying genome-wide signatures that define and drive specific neuronal fates has been hampered by enormous complexity in both time and space during development. Here, we have paired high-throughput purification of pyramidal neuron subclasses with deep profiling of spatiotemporal transcriptional dynamics during corticogenesis to resolve lineage choice decisions. We identified numerous features ranging from spatial and temporal usage of alternative mRNA isoforms and promoters to a host of mRNA genes modulated during fate specification. Notably, we uncovered numerous long noncoding RNAs with restricted temporal and cell-type-specific expression. To facilitate future exploration, we provide an interactive online database to enable multidimensional data mining and dissemination. This multifaceted study generates a powerful resource and informs understanding of the transcriptional regulation underlying pyramidal neuron diversity in the neocortex.

VIDEO ABSTRACT:

URLhttp://linkinghub.elsevier.com/retrieve/pii/S0896-6273(14)01133-7
DOI10.1016/j.neuron.2014.12.024
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/25556833?dopt=Abstract

Alternate JournalNeuron
PubMed ID25556833
PubMed Central IDPMC4430475
Grant ListDP2 OD006670 / OD / NIH HHS / United States
R01 NS078164 / NS / NINDS NIH HHS / United States
P01 GM099117 / GM / NIGMS NIH HHS / United States
R01 MH101268 / MH / NIMH NIH HHS / United States
F30 NS062489 / NS / NINDS NIH HHS / United States
R01 MH102416 / MH / NIMH NIH HHS / United States
NS073124 / NS / NINDS NIH HHS / United States
R01 NS073124 / NS / NINDS NIH HHS / United States
P50 HG006193 / HG / NHGRI NIH HHS / United States
P50 HG006193-01 / HG / NHGRI NIH HHS / United States
NIH R01 MH102416 / MH / NIMH NIH HHS / United States
DP2OD006670 / OD / NIH HHS / United States
NS078164 / NS / NINDS NIH HHS / United States
NS062489 / NS / NINDS NIH HHS / United States