You are here

iScience DOI:10.1016/j.isci.2020.101109

A PEROXO-Tag Enables Rapid Isolation of Peroxisomes from Human Cells.

Publication TypeJournal Article
Year of Publication2020
AuthorsG Ray, J, Boydston, EA, Shortt, E, Wyant, GA, Lourido, S, Chen, WW, Sabatini, DM
JournaliScience
Volume23
Issue5
Pages101109
Date Published2020 May 22
ISSN2589-0042
Abstract

Peroxisomes are metabolic organelles that perform a diverse array of critical functions in human physiology. Traditional isolation methods for peroxisomes can take more than 1 h to complete and can be laborious to implement. To address this, we have now extended our prior work on rapid organellar isolation to peroxisomes via the development of a peroxisomally localized 3XHA epitope tag ("PEROXO-Tag") and associated immunoprecipitation ("PEROXO-IP") workflow. Our PEROXO-IP workflow has excellent reproducibility, is easy to implement, and achieves highly rapid (~10 min post homogenization) and specific isolation of human peroxisomes, which we characterize here via proteomic profiling. By offering speed, specificity, reproducibility, and ease of use, the PEROXO-IP workflow should facilitate studies on the biology of peroxisomes.

DOI10.1016/j.isci.2020.101109
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/32417403?dopt=Abstract

Alternate JournaliScience
PubMed ID32417403