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Hum Mol Genet DOI:10.1093/hmg/ddz226

Annotations capturing cell type-specific TF binding explain a large fraction of disease heritability.

Publication TypeJournal Article
Year of Publication2020
Authorsvan de Geijn, B, Finucane, H, Gazal, S, Hormozdiari, F, Amariuta, T, Liu, X, Gusev, A, Loh, P-R, Reshef, Y, Kichaev, G, Raychauduri, S, Price, AL
JournalHum Mol Genet
Volume29
Issue7
Pages1057-1067
Date Published2020 May 08
ISSN1460-2083
Abstract

Regulatory variation plays a major role in complex disease and that cell type-specific binding of transcription factors (TF) is critical to gene regulation. However, assessing the contribution of genetic variation in TF-binding sites to disease heritability is challenging, as binding is often cell type-specific and annotations from directly measured TF binding are not currently available for most cell type-TF pairs. We investigate approaches to annotate TF binding, including directly measured chromatin data and sequence-based predictions. We find that TF-binding annotations constructed by intersecting sequence-based TF-binding predictions with cell type-specific chromatin data explain a large fraction of heritability across a broad set of diseases and corresponding cell types; this strategy of constructing annotations addresses both the limitation that identical sequences may be bound or unbound depending on surrounding chromatin context and the limitation that sequence-based predictions are generally not cell type-specific. We partitioned the heritability of 49 diseases and complex traits using stratified linkage disequilibrium (LD) score regression with the baseline-LD model (which is not cell type-specific) plus the new annotations. We determined that 100 bp windows around MotifMap sequenced-based TF-binding predictions intersected with a union of six cell type-specific chromatin marks (imputed using ChromImpute) performed best, with an 58% increase in heritability enrichment compared to the chromatin marks alone (11.6× vs. 7.3×, P = 9 × 10-14 for difference) and a 20% increase in cell type-specific signal conditional on annotations from the baseline-LD model (P = 8 × 10-11 for difference). Our results show that TF-binding annotations explain substantial disease heritability and can help refine genome-wide association signals.

DOI10.1093/hmg/ddz226
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/31595288?dopt=Abstract

Alternate JournalHum. Mol. Genet.
PubMed ID31595288
Grant ListR01 MH107649 / MH / NIMH NIH HHS / United States
R01 MH109978 / MH / NIMH NIH HHS / United States
R37 MH107649 / MH / NIMH NIH HHS / United States