You are here

Cell DOI:10.1016/j.cell.2020.04.018

Unblending of Transcriptional Condensates in Human Repeat Expansion Disease.

Publication TypeJournal Article
Year of Publication2020
AuthorsBasu, S, Mackowiak, SD, Niskanen, H, Knezevic, D, Asimi, V, Grosswendt, S, Geertsema, H, Ali, S, Jerković, I, Ewers, H, Mundlos, S, Meissner, A, Ibrahim, DM, Hnisz, D
JournalCell
Date Published2020 May 05
ISSN1097-4172
Abstract

Expansions of amino acid repeats occur in >20 inherited human disorders, and many occur in intrinsically disordered regions (IDRs) of transcription factors (TFs). Such diseases are associated with protein aggregation, but the contribution of aggregates to pathology has been controversial. Here, we report that alanine repeat expansions in the HOXD13 TF, which cause hereditary synpolydactyly in humans, alter its phase separation capacity and its capacity to co-condense with transcriptional co-activators. HOXD13 repeat expansions perturb the composition of HOXD13-containing condensates in vitro and in vivo and alter the transcriptional program in a cell-specific manner in a mouse model of synpolydactyly. Disease-associated repeat expansions in other TFs (HOXA13, RUNX2, and TBP) were similarly found to alter their phase separation. These results suggest that unblending of transcriptional condensates may underlie human pathologies. We present a molecular classification of TF IDRs, which provides a framework to dissect TF function in diseases associated with transcriptional dysregulation.

DOI10.1016/j.cell.2020.04.018
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/32386547?dopt=Abstract

Alternate JournalCell
PubMed ID32386547