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Nat Microbiol DOI:10.1038/s41564-019-0655-7

Growth effects of N-acylethanolamines on gut bacteria reflect altered bacterial abundances in inflammatory bowel disease.

Publication TypeJournal Article
Year of Publication2020
AuthorsFornelos, N, Franzosa, EA, Bishai, J, Annand, JW, Oka, A, Lloyd-Price, J, Arthur, TD, Garner, A, Avila-Pacheco, J, Haiser, HJ, Tolonen, AC, Porter, JA, Clish, CB, R Sartor, B, Huttenhower, C, Vlamakis, H, Xavier, RJ
JournalNat Microbiol
Date Published2020 Jan 20
ISSN2058-5276
Abstract

Inflammatory bowel diseases (IBD) are associated with alterations in gut microbial abundances and lumenal metabolite concentrations, but the effects of specific metabolites on the gut microbiota in health and disease remain largely unknown. Here, we analysed the influences of metabolites that are differentially abundant in IBD on the growth and physiology of gut bacteria that are also differentially abundant in IBD. We found that N-acylethanolamines (NAEs), a class of endogenously produced signalling lipids elevated in the stool of IBD patients and a T-cell transfer model of colitis, stimulated growth of species over-represented in IBD and inhibited that of species depleted in IBD in vitro. Using metagenomic sequencing, we recapitulated the effects of NAEs in complex microbial communities ex vivo, with Proteobacteria blooming and Bacteroidetes declining in the presence of NAEs. Metatranscriptomic analysis of the same communities identified components of the respiratory chain as important for the metabolism of NAEs, and this was verified using a mutant deficient for respiratory complex I. In this study, we identified NAEs as a class of metabolites that are elevated in IBD and have the potential to shift gut microbiota towards an IBD-like composition.

DOI10.1038/s41564-019-0655-7
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/31959971?dopt=Abstract

Alternate JournalNat Microbiol
PubMed ID31959971
Grant ListP01DK094779 / / U.S. Department of Health & Human Services | National Institutes of Health (NIH) /
P40OD010995 / / U.S. Department of Health & Human Services | National Institutes of Health (NIH) /
R24DK110499 / / U.S. Department of Health & Human Services | National Institutes of Health (NIH) /
U54DK102557 / / U.S. Department of Health & Human Services | National Institutes of Health (NIH) /
R24DK110499 / / U.S. Department of Health & Human Services | National Institutes of Health (NIH) /
U54DK102557 / / U.S. Department of Health & Human Services | National Institutes of Health (NIH) /
R01AT009708 / / U.S. Department of Health & Human Services | National Institutes of Health (NIH) /