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Elife DOI:10.7554/eLife.53003

STRIPAK directs PP2A activity toward MAP4K4 to promote oncogenic transformation of human cells.

Publication TypeJournal Article
Year of Publication2020
AuthorsKim, JWook, Berrios, C, Kim, M, Schade, AE, Adelmant, G, Yeerna, H, Damato, E, Iniguez, ABalboni, Florens, L, Washburn, MP, Stegmaier, K, Gray, NS, Tamayo, P, Gjoerup, O, Marto, JA, DeCaprio, J, Hahn, WC
JournalElife
Volume9
Date Published2020 Jan 08
ISSN2050-084X
Abstract

Alterations involving serine-threonine phosphatase PP2A subunits occur in a range of human cancers and partial loss of PP2A function contributes to cell transformation. Displacement of regulatory B subunits by the SV40 Small T antigen (ST) or mutation/deletion of PP2A subunits alters the abundance and types of PP2A complexes in cells, leading to transformation. Here we show that ST not only displaces common PP2A B subunits but also promotes A-C subunit interactions with alternative B subunits (B', striatins) that are components of the Striatin-interacting phosphatase and kinase (STRIPAK) complex. We found that STRN4, a member of STRIPAK, is associated with ST and is required for ST-PP2A-induced cell transformation. ST recruitment of STRIPAK facilitates PP2A-mediated dephosphorylation of MAP4K4 and induces cell transformation through the activation of the Hippo pathway effector YAP1. These observations identify an unanticipated role of MAP4K4 in transformation and show that the STRIPAK complex regulates PP2A specificity and activity.

DOI10.7554/eLife.53003
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/31913126?dopt=Abstract

Alternate JournalElife
PubMed ID31913126
Grant ListP01 CA203655 / CA / NCI NIH HHS / United States
U01 CA217885 / CA / NCI NIH HHS / United States