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Methods DOI:10.1016/j.ymeth.2014.10.033

Building the Connectivity Map of epigenetics: chromatin profiling by quantitative targeted mass spectrometry.

Publication TypeJournal Article
Year of Publication2015
AuthorsCreech, AL, Taylor, JE, Maier, VK, Wu, X, Feeney, CM, Udeshi, ND, Peach, SE, Boehm, JS, Lee, JT, Carr, SA, Jaffe, JD
Date Published2015 Jan 15
KeywordsChromatin, Epigenomics, Genomics, Histones, Humans, Mass Spectrometry, Protein Processing, Post-Translational, Proteomics

Epigenetic control of genome function is an important regulatory mechanism in diverse processes such as lineage commitment and environmental sensing, and in disease etiologies ranging from neuropsychiatric disorders to cancer. Here we report a robust, high-throughput targeted, quantitative mass spectrometry (MS) method to rapidly profile modifications of the core histones of chromatin that compose the epigenetic landscape, enabling comparisons among cells with differing genetic backgrounds, genomic perturbations, and drug treatments.


Alternate JournalMethods
PubMed ID25448295
PubMed Central IDPMC4300274
Grant ListR21 DA025720 / DA / NIDA NIH HHS / United States
U01 CA164186 / CA / NCI NIH HHS / United States
R21 DA025720-02 / DA / NIDA NIH HHS / United States
U01 CA164186-02 / CA / NCI NIH HHS / United States