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Cell DOI:10.1016/j.cell.2019.09.016

Optical Pooled Screens in Human Cells.

Publication TypeJournal Article
Year of Publication2019
AuthorsFeldman, D, Singh, A, Schmid-Burgk, JL, Carlson, RJ, Mezger, A, Garrity, AJ, Zhang, F, Blainey, PC
JournalCell
Volume179
Issue3
Pages787-799.e17
Date Published2019 Oct 17
ISSN1097-4172
Abstract

Genetic screens are critical for the systematic identification of genes underlying cellular phenotypes. Pooling gene perturbations greatly improves scalability but is not compatible with imaging of complex and dynamic cellular phenotypes. Here, we introduce a pooled approach for optical genetic screens in mammalian cells. We use targeted in situ sequencing to demultiplex a library of genetic perturbations following image-based phenotyping. We screened a set of 952 genes across millions of cells for involvement in nuclear factor κB (NF-κB) signaling by imaging the translocation of RelA (p65) to the nucleus. Screening at a single time point across 3 cell lines recovered 15 known pathway components, while repeating the screen with live-cell imaging revealed a role for Mediator complex subunits in regulating the duration of p65 nuclear retention. These results establish a highly multiplexed approach to image-based screens of spatially and temporally defined phenotypes with pooled libraries.

DOI10.1016/j.cell.2019.09.016
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/31626775?dopt=Abstract

Alternate JournalCell
PubMed ID31626775