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Nat Biotechnol DOI:10.1038/nbt.3070

A comparison of non-integrating reprogramming methods.

Publication TypeJournal Article
Year of Publication2015
AuthorsSchlaeger, TM, Daheron, L, Brickler, TR, Entwisle, S, Chan, K, Cianci, A, DeVine, A, Ettenger, A, Fitzgerald, K, Godfrey, M, Gupta, D, McPherson, J, Malwadkar, P, Gupta, M, Bell, B, Doi, A, Jung, N, Li, X, Lynes, MS, Brookes, E, Cherry, ABC, Demirbas, D, Tsankov, AM, Zon, LI, Rubin, LL, Feinberg, AP, Meissner, A, Cowan, CA, Daley, GQ
JournalNat Biotechnol
Volume33
Issue1
Pages58-63
Date Published2015 Jan
ISSN1546-1696
KeywordsCellular Reprogramming, Humans, Induced Pluripotent Stem Cells
Abstract

Human induced pluripotent stem cells (hiPSCs) are useful in disease modeling and drug discovery, and they promise to provide a new generation of cell-based therapeutics. To date there has been no systematic evaluation of the most widely used techniques for generating integration-free hiPSCs. Here we compare Sendai-viral (SeV), episomal (Epi) and mRNA transfection mRNA methods using a number of criteria. All methods generated high-quality hiPSCs, but significant differences existed in aneuploidy rates, reprogramming efficiency, reliability and workload. We discuss the advantages and shortcomings of each approach, and present and review the results of a survey of a large number of human reprogramming laboratories on their independent experiences and preferences. Our analysis provides a valuable resource to inform the use of specific reprogramming methods for different laboratories and different applications, including clinical translation.

DOI10.1038/nbt.3070
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/25437882?dopt=Abstract

Alternate JournalNat. Biotechnol.
PubMed ID25437882
PubMed Central IDPMC4329913
Grant ListP01 NS066888 / NS / NINDS NIH HHS / United States
R01HL75737 / HL / NHLBI NIH HHS / United States
R24DK092760 / DK / NIDDK NIH HHS / United States
U01-HL100001 / HL / NHLBI NIH HHS / United States
U01 HL100408 / HL / NHLBI NIH HHS / United States
F32 DK095537 / DK / NIDDK NIH HHS / United States
U01HL87402 / HL / NHLBI NIH HHS / United States
U01 HL100001 / HL / NHLBI NIH HHS / United States
R01 GM107536 / GM / NIGMS NIH HHS / United States
U01HL100408 / HL / NHLBI NIH HHS / United States
R24 DK092760 / DK / NIDDK NIH HHS / United States
R01 CA054358 / CA / NCI NIH HHS / United States
P01NS066888 / NS / NINDS NIH HHS / United States
U01HL107440 / HL / NHLBI NIH HHS / United States
U01 HL107440 / HL / NHLBI NIH HHS / United States