|Publication Type||Journal Article|
|Year of Publication||2019|
|Authors||Zamanighomi, M, Jain, SS, Ito, T, Pal, D, Daley, TP, Sellers, WR|
|Date Published||2019 07 12|
|Keywords||Bayes Theorem, Clustered Regularly Interspaced Short Palindromic Repeats, Epistasis, Genetic, Genetic Techniques, Software|
Systems for CRISPR-based combinatorial perturbation of two or more genes are emerging as powerful tools for uncovering genetic interactions. However, systematic identification of these relationships is complicated by sample, reagent, and biological variability. We develop a variational Bayes approach (GEMINI) that jointly analyzes all samples and reagents to identify genetic interactions in pairwise knockout screens. The improved accuracy and scalability of GEMINI enables the systematic analysis of combinatorial CRISPR knockout screens, regardless of design and dimension. GEMINI is available as an open source R package on GitHub at https://github.com/sellerslab/gemini .
|Alternate Journal||Genome Biol.|
|PubMed Central ID||PMC6624979|