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Nat Methods DOI:10.1038/s41592-019-0548-y

High-definition spatial transcriptomics for in situ tissue profiling.

Publication TypeJournal Article
Year of Publication2019
AuthorsVickovic, S, Eraslan, G, Salmén, F, Klughammer, J, Stenbeck, L, Schapiro, D, Äijö, T, Bonneau, R, Bergenstråhle, L, Navarro, JFernandéz, Gould, J, Griffin, GK, Borg, A, Ronaghi, M, Frisén, J, Lundeberg, J, Regev, A, Ståhl, PL
JournalNat Methods
Date Published2019 Sep 09
ISSN1548-7105
Abstract

Spatial and molecular characteristics determine tissue function, yet high-resolution methods to capture both concurrently are lacking. Here, we developed high-definition spatial transcriptomics, which captures RNA from histological tissue sections on a dense, spatially barcoded bead array. Each experiment recovers several hundred thousand transcript-coupled spatial barcodes at 2-μm resolution, as demonstrated in mouse brain and primary breast cancer. This opens the way to high-resolution spatial analysis of cells and tissues.

DOI10.1038/s41592-019-0548-y
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/31501547?dopt=Abstract

Alternate JournalNat. Methods
PubMed ID31501547
Grant List2016.0454 / / Knut och Alice Wallenbergs Stiftelse (Knut and Alice Wallenberg Foundation) /
ALTF 738-2017 / / European Molecular Biology Organization (EMBO) /
1OT2OD026673-1 / / U.S. Department of Health & Human Services | National Institutes of Health (NIH) /
P2ZHP3_181475 / / Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation) /