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Cancer Discov DOI:10.1158/2159-8290.CD-13-1037

The genomic landscape of pediatric Ewing sarcoma.

Publication TypeJournal Article
Year of Publication2014
AuthorsCrompton, BD, Stewart, C, Taylor-Weiner, A, Alexe, G, Kurek, KC, Calicchio, ML, Kiezun, A, Carter, SL, Shukla, SA, Mehta, SS, Thorner, AR, de Torres, C, Lavarino, C, Suñol, M, McKenna, A, Sivachenko, A, Cibulskis, K, Lawrence, MS, Stojanov, P, Rosenberg, M, Ambrogio, L, Auclair, D, Seepo, S, Blumenstiel, B, DeFelice, M, Imaz-Rosshandler, I, Schwarz-Cruz y Celis, A, Rivera, MN, Rodriguez-Galindo, C, Fleming, MD, Golub, TR, Getz, G, Mora, J, Stegmaier, K
JournalCancer Discov
Volume4
Issue11
Pages1326-41
Date Published2014 Nov
ISSN2159-8290
KeywordsAntigens, Nuclear, Bone Neoplasms, Cell Line, Tumor, Child, DNA, Neoplasm, Female, Gene Rearrangement, Genomics, Humans, Male, Mutation, Sarcoma, Ewing, Sequence Analysis, DNA
Abstract

UNLABELLED: Pediatric Ewing sarcoma is characterized by the expression of chimeric fusions of EWS and ETS family transcription factors, representing a paradigm for studying cancers driven by transcription factor rearrangements. In this study, we describe the somatic landscape of pediatric Ewing sarcoma. These tumors are among the most genetically normal cancers characterized to date, with only EWS-ETS rearrangements identified in the majority of tumors. STAG2 loss, however, is present in more than 15% of Ewing sarcoma tumors; occurs by point mutation, rearrangement, and likely nongenetic mechanisms; and is associated with disease dissemination. Perhaps the most striking finding is the paucity of mutations in immediately targetable signal transduction pathways, highlighting the need for new therapeutic approaches to target EWS-ETS fusions in this disease.

SIGNIFICANCE: We performed next-generation sequencing of Ewing sarcoma, a pediatric cancer involving bone, characterized by expression of EWS-ETS fusions. We found remarkably few mutations. However, we discovered that loss of STAG2 expression occurs in 15% of tumors and is associated with metastatic disease, suggesting a potential genetic vulnerability in Ewing sarcoma.

URLhttp://cancerdiscovery.aacrjournals.org/cgi/pmidlookup?view=long&pmid=25186949
DOI10.1158/2159-8290.CD-13-1037
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/25186949?dopt=Abstract

Alternate JournalCancer Discov
PubMed ID25186949