Gabapentin attenuates hyperexcitability in the freeze-lesion model of developmental cortical malformation.
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Abstract | Developmental cortical malformations are associated with a high incidence of drug-resistant epilepsy. The underlying epileptogenic mechanisms, however, are poorly understood. In rodents, cortical malformations can be modeled using neonatal freeze-lesion (FL), which has been shown to cause in vitro cortical hyperexcitability. Here, we investigated the therapeutic potential of gabapentin, a clinically used anticonvulsant and analgesic, in preventing FL-induced in vitro and in vivo hyperexcitability. Gabapentin has been shown to disrupt the interaction of thrombospondin (TSP) with α2δ-1, an auxiliary calcium channel subunit. TSP/α2δ-1 signaling has been shown to drive the formation of excitatory synapses during cortical development and following injury. Gabapentin has been reported to have neuroprotective and anti-epileptogenic effects in other models associated with increased TSP expression and reactive astrocytosis. We found that both TSP and α2δ-1 were transiently upregulated following neonatal FL. We therefore designed a one-week GBP treatment paradigm to block TSP/α2δ-1 signaling during the period of their upregulation. GBP treatment prevented epileptiform activity following FL, as assessed by both glutamate biosensor imaging and field potential recording. GBP also attenuated FL-induced increases in mEPSC frequency at both P7 and 28. Additionally, GBP treated animals had decreased in vivo kainic acid (KA)-induced seizure activity. Taken together these results suggest gabapentin treatment immediately after FL can prevent the formation of a hyperexcitable network and may have therapeutic potential to minimize epileptogenic processes associated with developmental cortical malformations. |
Year of Publication | 2014
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Journal | Neurobiol Dis
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Volume | 71
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Pages | 305-16
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Date Published | 2014 Nov
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ISSN | 1095-953X
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DOI | 10.1016/j.nbd.2014.08.022
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PubMed ID | 25158291
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PubMed Central ID | PMC4179994
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Grant list | R01-NS073574 / NS / NINDS NIH HHS / United States
R01 MH099554 / MH / NIMH NIH HHS / United States
R01-MH099554 / MH / NIMH NIH HHS / United States
R01 NS076885 / NS / NINDS NIH HHS / United States
P30 NS047243 / NS / NINDS NIH HHS / United States
R01-NS076885 / NS / NINDS NIH HHS / United States
R01 NS073574 / NS / NINDS NIH HHS / United States
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