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ACS Chem Biol DOI:10.1021/cb500492r

An unbiased approach to identify endogenous substrates of "histone" deacetylase 8.

Publication TypeJournal Article
Year of Publication2014
AuthorsOlson, DE, Udeshi, ND, Wolfson, NA, Pitcairn, CAnn, Sullivan, ED, Jaffe, JD, Svinkina, T, Natoli, T, Lu, X, Paulk, J, McCarren, P, Wagner, FF, Barker, D, Howe, E, Lazzaro, F, Gale, JP, Zhang, Y-L, Subramanian, A, Fierke, CA, Carr, SA, Holson, EB
JournalACS Chem Biol
Volume9
Issue10
Pages2210-6
Date Published2014 Oct 17
ISSN1554-8937
KeywordsAcetylation, Histone Deacetylase Inhibitors, Histone Deacetylases, Humans, Nuclear Proteins, Protein Interaction Maps, Proteomics, Repressor Proteins, Substrate Specificity, Transcription Factors
Abstract

Despite being extensively characterized structurally and biochemically, the functional role of histone deacetylase 8 (HDAC8) has remained largely obscure due in part to a lack of known cellular substrates. Herein, we describe an unbiased approach using chemical tools in conjunction with sophisticated proteomics methods to identify novel non-histone nuclear substrates of HDAC8, including the tumor suppressor ARID1A. These newly discovered substrates of HDAC8 are involved in diverse biological processes including mitosis, transcription, chromatin remodeling, and RNA splicing and may help guide therapeutic strategies that target the function of HDAC8.

URLhttp://dx.doi.org/10.1021/cb500492r
DOI10.1021/cb500492r
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/25089360?dopt=Abstract

Alternate JournalACS Chem. Biol.
PubMed ID25089360
PubMed Central IDPMC4201337
Grant ListGM40602 / GM / NIGMS NIH HHS / United States
U24 CA160034 / CA / NCI NIH HHS / United States
T32 GM008353 / GM / NIGMS NIH HHS / United States
R01HL096738 / HL / NHLBI NIH HHS / United States
R01 HL096738 / HL / NHLBI NIH HHS / United States
HHSN268201000033C / / PHS HHS / United States
U54 HG006093 / HG / NHGRI NIH HHS / United States
R01 GM040602 / GM / NIGMS NIH HHS / United States
T32-GM-008597 / GM / NIGMS NIH HHS / United States
HHSN268201000033C / HL / NHLBI NIH HHS / United States
T32-GM-008353 / GM / NIGMS NIH HHS / United States
T32 GM008597 / GM / NIGMS NIH HHS / United States
U24CA160034 / CA / NCI NIH HHS / United States