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Nat Methods DOI:10.1038/nmeth.3063

Efficient CRISPR-Cas9-mediated genome editing in Plasmodium falciparum.

Publication TypeJournal Article
Year of Publication2014
AuthorsWagner, JC, Platt, RJ, Goldfless, SJ, Zhang, F, Niles, JC
JournalNat Methods
Date Published2014 Sep
KeywordsAnimals, Animals, Genetically Modified, Base Sequence, CRISPR-Cas Systems, Genetic Engineering, Genome, Molecular Sequence Data, Plasmodium falciparum, RNA Editing

Malaria is a major cause of global morbidity and mortality, and new strategies for treating and preventing this disease are needed. Here we show that the Streptococcus pyogenes Cas9 DNA endonuclease and single guide RNAs (sgRNAs) produced using T7 RNA polymerase (T7 RNAP) efficiently edit the Plasmodium falciparum genome. Targeting the genes encoding native knob-associated histidine-rich protein (kahrp) and erythrocyte binding antigen 175 (eba-175), we achieved high (≥ 50-100%) gene disruption frequencies within the usual time frame for generating transgenic parasites.


Alternate JournalNat. Methods
PubMed ID25108687
PubMed Central IDPMC4199390
Grant List1DP2OD007124 / OD / NIH HHS / United States
DP2 OD007124 / OD / NIH HHS / United States
5-T32-ES007020 / ES / NIEHS NIH HHS / United States
T32 GM008334 / GM / NIGMS NIH HHS / United States
T32 ES007020 / ES / NIEHS NIH HHS / United States
P30 ES002109 / ES / NIEHS NIH HHS / United States
DP1 MH100706 / MH / NIMH NIH HHS / United States
R01 DK097768 / DK / NIDDK NIH HHS / United States
5-T32-GM08334 / GM / NIGMS NIH HHS / United States
P30-ES002109 / ES / NIEHS NIH HHS / United States