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Nature methods DOI:10.1038/nmeth.3063

Efficient CRISPR-Cas9-mediated genome editing in Plasmodium falciparum.

Publication TypeJournal Article
Year of Publication2014
AuthorsWagner, JC, Platt, RJ, Goldfless, SJ, Zhang, F, Niles, JC
JournalNature methods
Date Published2014/08/10

Malaria is a major cause of global morbidity and mortality, and new strategies for treating and preventing this disease are needed. Here we show that the Streptococcus pyogenes Cas9 DNA endonuclease and single guide RNAs (sgRNAs) produced using T7 RNA polymerase (T7 RNAP) efficiently edit the Plasmodium falciparum genome. Targeting the genes encoding native knob-associated histidine-rich protein (kahrp) and erythrocyte binding antigen 175 (eba-175), we achieved high (≥50-100%) gene disruption frequencies within the usual time frame for generating transgenic parasites.