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Hum Mol Genet DOI:10.1093/hmg/ddu379

De novo CNVs in bipolar affective disorder and schizophrenia.

Publication TypeJournal Article
Year of Publication2014
AuthorsGeorgieva, L, Rees, E, Moran, JL, Chambert, KD, Milanova, V, Craddock, N, Purcell, S, Sklar, P, McCarroll, S, Holmans, P, O'Donovan, MC, Owen, MJ, Kirov, G
JournalHum Mol Genet
Volume23
Issue24
Pages6677-83
Date Published2014 Dec 15
ISSN1460-2083
KeywordsAdolescent, Adult, Algorithms, Bipolar Disorder, Case-Control Studies, Child, Chromosomes, Human, Pair 16, DNA Copy Number Variations, Female, Genetic Loci, Genetic Predisposition to Disease, Genome-Wide Association Study, Genotyping Techniques, Humans, Male, Middle Aged, Oligonucleotide Array Sequence Analysis, Pedigree, Schizophrenia
Abstract

An increased rate of de novo copy number variants (CNVs) has been found in schizophrenia (SZ), autism and developmental delay. An increased rate has also been reported in bipolar affective disorder (BD). Here, in a larger BD sample, we aimed to replicate these findings and compare de novo CNVs between SZ and BD. We used Illumina microarrays to genotype 368 BD probands, 76 SZ probands and all their parents. Copy number variants were called by PennCNV and filtered for frequency (10 kb). Putative de novo CNVs were validated with the z-score algorithm, manual inspection of log R ratios (LRR) and qPCR probes. We found 15 de novo CNVs in BD (4.1% rate) and 6 in SZ (7.9% rate). Combining results with previous studies and using a cut-off of >100 kb, the rate of de novo CNVs in BD was intermediate between controls and SZ: 1.5% in controls, 2.2% in BD and 4.3% in SZ. Only the differences between SZ and BD and SZ and controls were significant. The median size of de novo CNVs in BD (448 kb) was also intermediate between SZ (613 kb) and controls (338 kb), but only the comparison between SZ and controls was significant. Only one de novo CNV in BD was in a confirmed SZ locus (16p11.2). Sporadic or early onset cases were not more likely to have de novo CNVs. We conclude that de novo CNVs play a smaller role in BD compared with SZ. Patients with a positive family history can also harbour de novo mutations.

URLhttp://hmg.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=25055870
DOI10.1093/hmg/ddu379
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/25055870?dopt=Abstract

Alternate JournalHum. Mol. Genet.
PubMed ID25055870
PubMed Central IDPMC4240207
Grant ListG0801418 / / Medical Research Council / United Kingdom
MR/L010305/1 / / Medical Research Council / United Kingdom
G0800509 / / Medical Research Council / United Kingdom
/ / Wellcome Trust / United Kingdom