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Journal of microbiological methods DOI:10.1016/j.mimet.2014.05.018

Selection of unique Escherichia coli clones by random amplified polymorphic DNA (RAPD): Evaluation by whole genome sequencing.

Publication TypeJournal Article
Year of Publication2014
AuthorsW Fricke, F, Godfrey, PA, Giovanni, M, Hine, E, Feldgarden, M, Hotopp, JDunning
JournalJournal of microbiological methods
Volume103
Pages101-3
Date Published2014/08/01
ISSN0167-7012
Abstract

Identifying and characterizing clonal diversity are important when analysing fecal flora. We evaluated random amplified polymorphic DNA (RAPD) PCR, applied for selection of Escherichia coli isolates, by whole genome sequencing. RAPD was fast, and reproducible as screening method for selection of distinct E. coli clones in fecal swabs.

URLhttp://linkinghub.elsevier.com/retrieve/pii/S0167-7012(14)00148-1
DOI10.1016/j.mimet.2014.05.018
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/24912108?dopt=Abstract