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J Microbiol Methods DOI:10.1016/j.mimet.2014.05.018

Selection of unique Escherichia coli clones by random amplified polymorphic DNA (RAPD): Evaluation by whole genome sequencing.

Publication TypeJournal Article
Year of Publication2014
AuthorsNielsen, KL, Godfrey, PA, Stegger, M, Andersen, PS, Feldgarden, M, Frimodt-Møller, N
JournalJ Microbiol Methods
Date Published2014 Aug
KeywordsEscherichia coli, Escherichia coli Infections, Feces, Genome, Bacterial, High-Throughput Nucleotide Sequencing, Molecular Typing, Random Amplified Polymorphic DNA Technique, Reproducibility of Results

Identifying and characterizing clonal diversity are important when analysing fecal flora. We evaluated random amplified polymorphic DNA (RAPD) PCR, applied for selection of Escherichia coli isolates, by whole genome sequencing. RAPD was fast, and reproducible as screening method for selection of distinct E. coli clones in fecal swabs.


Alternate JournalJ. Microbiol. Methods
PubMed ID24912108
PubMed Central IDPMC4166437
Grant ListHHSN272200900018C / AI / NIAID NIH HHS / United States
U19 AI110818 / AI / NIAID NIH HHS / United States
HHSN272200900018C / / PHS HHS / United States