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Curr Opin Chem Eng DOI:10.1016/j.coche.2014.01.011

Towards Engineered Processes for Sequencing-Based Analysis of Single Circulating Tumor Cells.

Publication TypeJournal Article
Year of Publication2014
AuthorsAdalsteinsson, VA, J Love, C
JournalCurr Opin Chem Eng
Date Published2014 May 01

Sequencing-based analysis of single circulating tumor cells (CTCs) has the potential to revolutionize our understanding of metastatic cancer and improve clinical care. Technologies exist to enrich, identify, recover, and sequence single cells, but to enable systematic routine analysis of single CTCs from a range of cancer patients, there is a need to establish processes that efficiently integrate these specific operations. Such engineered processes should address challenges associated with the yield and viability of enriched CTCs, the robust identification of candidate single CTCs with minimal degradation of DNA, the bias in whole-genome amplification, and the efficient handling of candidate single CTCs or their amplified DNA products. Advances in methods for single-cell analysis and nanoscale technologies suggest opportunities to overcome these challenges, and could create integrated platforms that perform several of the unit operations together. Ultimately, technologies should be selected or adapted for optimal performance and compatibility in an integrated process.


Alternate JournalCurr Opin Chem Eng
PubMed ID24839591
PubMed Central IDPMC4019410
Grant ListP30 CA014051 / CA / NCI NIH HHS / United States