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Int J Syst Evol Microbiol DOI:10.1099/ijs.0.060988-0

Oribacterium parvum sp. nov. and Oribacterium asaccharolyticum sp. nov., obligately anaerobic bacteria from the human oral cavity, and emended description of the genus Oribacterium.

Publication TypeJournal Article
Year of Publication2014
AuthorsSizova, MV, Muller, PA, Stancyk, D, Panikov, NS, Mandalakis, M, Hazen, A, Hohmann, T, Doerfert, SN, Fowle, W, Earl, AM, Nelson, KE, Epstein, SS
JournalInt J Syst Evol Microbiol
IssuePt 8
Date Published2014 Aug
KeywordsAdult, Bacteria, Anaerobic, Bacterial Typing Techniques, Base Composition, Dental Plaque, Diaminopimelic Acid, DNA, Bacterial, Fatty Acids, Female, Gram-Positive Asporogenous Rods, Humans, Molecular Sequence Data, Mouth, Nucleic Acid Hybridization, Peptidoglycan, Phylogeny, RNA, Ribosomal, 16S, Sequence Analysis, DNA

Three strictly anaerobic, Gram-positive, non-spore-forming, rod-shaped, motile bacteria, designated strains ACB1(T), ACB7(T) and ACB8, were isolated from human subgingival dental plaque. All strains required yeast extract for growth. Strains ACB1(T) and ACB8 were able to grow on glucose, lactose, maltose, maltodextrin and raffinose; strain ACB7(T) grew weakly on sucrose only. The growth temperature range was 30-42 °C with optimum growth at 37 °C. Major metabolic fermentation end products of strain ACB1(T) were acetate and lactate; the only product of strains ACB7(T) and ACB8 was acetate. Major fatty acids of strain ACB1(T) were C(14 : 0), C(16 : 0), C(16 : 1)ω7c dimethyl aldehyde (DMA) and C(18 : 1)ω7c DMA. Major fatty acids of strain ACB7(T) were C(12 : 0), C(14 : 0), C(16 : 0), C(16 : 1)ω7c and C(16 : 1)ω7c DMA. The hydrolysate of the peptidoglycan contained meso-diaminopimelic acid, indicating peptidoglycan type A1γ. Genomic DNA G+C content varied from 42 to 43.3% between strains. According to 16S rRNA gene sequence phylogeny, strains ACB1(T), ACB8 and ACB7(T) formed two separate branches within the genus Oribacterium, with 98.1-98.6% sequence similarity to the type strain of the type species, Oribacterium sinus. Predicted DNA-DNA hybridization values between strains ACB1(T), ACB8, ACB7(T) and O. sinus F0268 were <70%. Based on distinct genotypic and phenotypic characteristics, strains ACB1(T) and ACB8, and strain ACB7(T) are considered to represent two distinct species of the genus Oribacterium, for which the names Oribacterium parvum sp. nov. and Oribacterium asaccharolyticum sp. nov. are proposed. The type strains are ACB1(T) ( = DSM 24637(T) = HM-481(T) = ATCC BAA-2638(T)) and ACB7(T) ( = DSM 24638(T) = HM-482(T) = ATCC BAA-2639(T)), respectively.


Alternate JournalInt. J. Syst. Evol. Microbiol.
PubMed ID24824639
PubMed Central IDPMC4129163
Grant ListU54HG004969 / HG / NHGRI NIH HHS / United States
U54 AI084844 / AI / NIAID NIH HHS / United States
1U54 AI84844-01 / AI / NIAID NIH HHS / United States
R21 DE018026 / DE / NIDCR NIH HHS / United States
1RC1DE020707-01 / DE / NIDCR NIH HHS / United States
U54 HG004969 / HG / NHGRI NIH HHS / United States
RC1 DE020707 / DE / NIDCR NIH HHS / United States
3 R21 DE018026-02S1 / DE / NIDCR NIH HHS / United States