Constant growth rate can be supported by decreasing energy flux and increasing aerobic glycolysis.
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Abstract | Fermenting glucose in the presence of enough oxygen to support respiration, known as aerobic glycolysis, is believed to maximize growth rate. We observed increasing aerobic glycolysis during exponential growth, suggesting additional physiological roles for aerobic glycolysis. We investigated such roles in yeast batch cultures by quantifying O2 consumption, CO2 production, amino acids, mRNAs, proteins, posttranslational modifications, and stress sensitivity in the course of nine doublings at constant rate. During this course, the cells support a constant biomass-production rate with decreasing rates of respiration and ATP production but also decrease their stress resistance. As the respiration rate decreases, so do the levels of enzymes catalyzing rate-determining reactions of the tricarboxylic-acid cycle (providing NADH for respiration) and of mitochondrial folate-mediated NADPH production (required for oxidative defense). The findings demonstrate that exponential growth can represent not a single metabolic/physiological state but a continuum of changing states and that aerobic glycolysis can reduce the energy demands associated with respiratory metabolism and stress survival. |
Year of Publication | 2014
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Journal | Cell Rep
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Volume | 7
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Issue | 3
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Pages | 705-14
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Date Published | 2014 May 08
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ISSN | 2211-1247
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URL | |
DOI | 10.1016/j.celrep.2014.03.057
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PubMed ID | 24767987
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PubMed Central ID | PMC4049626
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Grant list | DP1 CA174420 / CA / NCI NIH HHS / United States
R01 GM096193 / GM / NIGMS NIH HHS / United States
R01-GM-096193 / GM / NIGMS NIH HHS / United States
U54 CA143874 / CA / NCI NIH HHS / United States
R01 GM068957 / GM / NIGMS NIH HHS / United States
U54CA143874 / CA / NCI NIH HHS / United States
DP1 OD003936 / OD / NIH HHS / United States
R01-GM068957 / GM / NIGMS NIH HHS / United States
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