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Cell Rep DOI:10.1016/j.celrep.2014.03.057

Constant growth rate can be supported by decreasing energy flux and increasing aerobic glycolysis.

Publication TypeJournal Article
Year of Publication2014
AuthorsSlavov, N, Budnik, BA, Schwab, D, Airoldi, EM, van Oudenaarden, A
JournalCell Rep
Date Published2014 May 08
KeywordsAdenosine Triphosphate, Batch Cell Culture Techniques, Biomass, Citric Acid Cycle, Energy Metabolism, Glycolysis, Mitochondria, NADP, Protein Processing, Post-Translational, RNA, Messenger, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors

Fermenting glucose in the presence of enough oxygen to support respiration, known as aerobic glycolysis, is believed to maximize growth rate. We observed increasing aerobic glycolysis during exponential growth, suggesting additional physiological roles for aerobic glycolysis. We investigated such roles in yeast batch cultures by quantifying O2 consumption, CO2 production, amino acids, mRNAs, proteins, posttranslational modifications, and stress sensitivity in the course of nine doublings at constant rate. During this course, the cells support a constant biomass-production rate with decreasing rates of respiration and ATP production but also decrease their stress resistance. As the respiration rate decreases, so do the levels of enzymes catalyzing rate-determining reactions of the tricarboxylic-acid cycle (providing NADH for respiration) and of mitochondrial folate-mediated NADPH production (required for oxidative defense). The findings demonstrate that exponential growth can represent not a single metabolic/physiological state but a continuum of changing states and that aerobic glycolysis can reduce the energy demands associated with respiratory metabolism and stress survival.


Alternate JournalCell Rep
PubMed ID24767987
PubMed Central IDPMC4049626
Grant ListDP1 CA174420 / CA / NCI NIH HHS / United States
R01 GM096193 / GM / NIGMS NIH HHS / United States
R01-GM-096193 / GM / NIGMS NIH HHS / United States
U54 CA143874 / CA / NCI NIH HHS / United States
R01 GM068957 / GM / NIGMS NIH HHS / United States
U54CA143874 / CA / NCI NIH HHS / United States
DP1 OD003936 / OD / NIH HHS / United States
R01-GM068957 / GM / NIGMS NIH HHS / United States