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Nat Commun DOI:10.1038/ncomms4728

Targeted genomic rearrangements using CRISPR/Cas technology.

Publication TypeJournal Article
Year of Publication2014
AuthorsChoi, PS, Meyerson, M
JournalNat Commun
Volume5
Pages3728
Date Published2014 Apr 24
ISSN2041-1723
KeywordsClustered Regularly Interspaced Short Palindromic Repeats, Gene Rearrangement, Humans
Abstract

Genomic rearrangements are frequently observed in cancer cells but have been difficult to generate in a highly specific manner for functional analysis. Here we report the application of CRISPR/Cas technology to successfully generate several types of chromosomal rearrangements implicated as driver events in lung cancer, including the CD74-ROS1 translocation event and the EML4-ALK and KIF5B-RET inversion events. Our results demonstrate that Cas9-induced DNA breaks promote efficient rearrangement between pairs of targeted loci, providing a highly tractable approach for the study of genomic rearrangements.

URLhttp://dx.doi.org/10.1038/ncomms4728
DOI10.1038/ncomms4728
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/24759083?dopt=Abstract

Alternate JournalNat Commun
PubMed ID24759083
PubMed Central IDPMC4170920
Grant ListR01 CA109038 / CA / NCI NIH HHS / United States
NCI R01CA109038 / CA / NCI NIH HHS / United States