You are here

PLoS One DOI:10.1371/journal.pone.0094187

ZBED6 modulates the transcription of myogenic genes in mouse myoblast cells.

Publication TypeJournal Article
Year of Publication2014
AuthorsJiang, L, Wallerman, O, Younis, S, Rubin, C-J, Gilbert, ER, Sundström, E, Ghazal, A, Zhang, X, Wang, L, Mikkelsen, TS, Andersson, G, Andersson, L
JournalPLoS One
Date Published2014
KeywordsAnimals, Histones, Mice, Muscle Proteins, Myoblasts, Promoter Regions, Genetic, Repressor Proteins, Transcription, Genetic

ZBED6 is a recently discovered transcription factor, unique to placental mammals, that has evolved from a domesticated DNA transposon. It acts as a repressor at the IGF2 locus. Here we show that ZBED6 acts as a transcriptional modulator in mouse myoblast cells, where more than 700 genes were differentially expressed after Zbed6-silencing. The most significantly enriched GO term was muscle protein and contractile fiber, which was consistent with increased myotube formation. Twenty small nucleolar RNAs all showed increased expression after Zbed6-silencing. The co-localization of histone marks and ZBED6 binding sites and the effect of Zbed6-silencing on distribution of histone marks was evaluated by ChIP-seq analysis. There was a strong association between ZBED6 binding sites and the H3K4me3, H3K4me2 and H3K27ac modifications, which are usually found at active promoters, but no association with the repressive mark H3K27me3. Zbed6-silencing led to increased enrichment of active marks at myogenic genes, in agreement with the RNA-seq findings. We propose that ZBED6 preferentially binds to active promoters and modulates transcriptional activity without recruiting repressive histone modifications.


Alternate JournalPLoS ONE
PubMed ID24714595
PubMed Central IDPMC3979763