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Genome Biol DOI:10.1186/gb-2012-13-10-r85

H2A.Z landscapes and dual modifications in pluripotent and multipotent stem cells underlie complex genome regulatory functions.

Publication TypeJournal Article
Year of Publication2012
AuthorsKu, M, Jaffe, JD, Koche, RP, Rheinbay, E, Endoh, M, Koseki, H, Carr, SA, Bernstein, BE
JournalGenome Biol
Date Published2012 Oct 03
KeywordsAnimals, Chromatin, Embryonic Stem Cells, Enhancer Elements, Genetic, Genome, Histones, Humans, Mice, Multipotent Stem Cells, Neural Stem Cells, Pluripotent Stem Cells, Promoter Regions, Genetic, Protein Processing, Post-Translational, Transcriptional Activation

BACKGROUND: The histone variant H2A.Z has been implicated in nucleosome exchange, transcriptional activation and Polycomb repression. However, the relationships among these seemingly disparate functions remain obscure.

RESULTS: We mapped H2A.Z genome-wide in mammalian ES cells and neural progenitors. H2A.Z is deposited promiscuously at promoters and enhancers, and correlates strongly with H3K4 methylation. Accordingly, H2A.Z is present at poised promoters with bivalent chromatin and at active promoters with H3K4 methylation, but is absent from stably repressed promoters that are specifically enriched for H3K27 trimethylation. We also characterized post-translational modification states of H2A.Z, including a novel species dually-modified by ubiquitination and acetylation that is enriched at bivalent chromatin.

CONCLUSIONS: Our findings associate H2A.Z with functionally distinct genomic elements, and suggest that post-translational modifications may reconcile its contrasting locations and roles.


Alternate JournalGenome Biol.
PubMed ID23034477
PubMed Central IDPMC3491413