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Stem Cell Reports DOI:10.1016/j.stemcr.2014.01.007

In Vivo and in vitro dynamics of undifferentiated embryonic cell transcription factor 1.

Publication TypeJournal Article
Year of Publication2014
AuthorsGalonska, C, Smith, ZD, Meissner, A
JournalStem Cell Reports
Volume2
Issue3
Pages245-52
Date Published2014 Mar 11
KeywordsAnimals, Biomarkers, Cell Differentiation, Cell Line, Cellular Reprogramming, Chromosomal Proteins, Non-Histone, DNA, Embryo, Mammalian, Embryonic Stem Cells, Gene Expression, Gene Expression Regulation, Developmental, Gene Order, Genes, Reporter, Genetic Vectors, Mice, Pluripotent Stem Cells, Protein Binding, Trans-Activators, Transcription Factors
Abstract

Pluripotent stem cells retain the ability to differentiate into the three germ layers and germline. As a result, there is a major interest in characterizing regulators that establish and maintain pluripotency. The network of transcription factors continues to expand in complexity, and one factor, undifferentiated embryonic cell transcription factor 1 (UTF1), has recently moved more into the limelight. To facilitate the study of UTF1, we report the generation and characterization of two reporter lines that enable efficient tracking, mapping, and purification of endogenous UTF1. In particular, we include a built-in biotinylation system in our targeted locus that allows efficient and reliable pulldown. We also use this reporter to show the dynamic regulation of Utf1 in distinct stem cell conditions and demonstrate its utility for reprogramming studies. The multipurpose design of the reporter lines enables many directions of future study and should lead to a better understanding of UTF1's diverse roles.

DOI10.1016/j.stemcr.2014.01.007
Pubmed

http://www.ncbi.nlm.nih.gov/pubmed/24672748?dopt=Abstract

Alternate JournalStem Cell Reports
PubMed ID24672748
PubMed Central IDPMC3964277
Grant ListP01 GM099117 / GM / NIGMS NIH HHS / United States
P50 HG006193 / HG / NHGRI NIH HHS / United States
1P50HG006193 / HG / NHGRI NIH HHS / United States
P01GM099117 / GM / NIGMS NIH HHS / United States